A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells

Chemical Communications : Chem Comm
Naseer Ullah KhanFang Huang

Abstract

An ultra-high fluorescence enhancement for two dyes on photonic crystal films was achieved to construct a two-color immuno-dot blot assay. This assay was demonstrated to simultaneously detect chemokine receptor co-expressed in cancer cells and reveal their co-operative and subtle changes after binding with respective ligands and drugs.

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Citations

May 11, 2021·Chemical Communications : Chem Comm·Qian WangFang Huang
Jun 17, 2021·Analytical Chemistry·Qian WangFang Huang

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
transmission electron microscopy
fluorescence imaging
sandwich immunoassay
dot-blot
Fluorescence

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