PMID: 9171411Jun 1, 1997Paper

A two-component system in Ralstonia (Pseudomonas) solanacearum modulates production of PhcA-regulated virulence factors in response to 3-hydroxypalmitic acid methyl ester

Journal of Bacteriology
S J CloughTimothy P Denny

Abstract

Expression of virulence factors in Ralstonia solanacearum is controlled by a complex regulatory network, at the center of which is PhcA, a LysR family transcriptional regulator. We report here that expression of phcA and production of PhcA-regulated virulence factors are affected by products of the putative operon phcBSR(Q). phcB is required for production of an extracellular factor (EF), tentatively identified as the fatty acid derivative 3-hydroxypalmitic acid methyl ester (3-OH PAME), but a biochemical function for PhcB could not be deduced from DNA sequence analysis. The other genes in the putative operon are predicted to encode proteins homologous to members of two-component signal transduction systems: PhcS has amino acid similarity to histidine kinase sensors, whereas PhcR and OrfQ are similar to response regulators. PhcR is quite unusual because its putative output domain strongly resembles the histidine kinase domain of a sensor protein. Production of the PhcA-regulated factors exopolysaccharide I, endoglucanase, and pectin methyl esterase was reduced 10- to 100-fold only in mutants with a nonpolar insertion in phcB [which express phcSR(Q) in the absence of the EF]; simultaneously, expression of phcA was reduced fivefo...Continue Reading

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Citations

Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Julie Tans-KerstenCaitilyn Allen
Oct 19, 2006·Analytical and Bioanalytical Chemistry·Alexander R HorswillMatthew R Parsek
Mar 23, 2011·Journal of Bacteriology·Fanhong MengCaitilyn Allen
Sep 12, 2015·Chembiochem : a European Journal of Chemical Biology·Kenji KaiYasufumi Hikichi
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Feb 10, 2018·Bioscience, Biotechnology, and Biochemistry·Kenji Kai
Oct 27, 2006·FEMS Microbiology Ecology·Ludovic VialFlorence Wisniewski-Dyé
Dec 10, 2015·Bioscience, Biotechnology, and Biochemistry·Kenji KaiYasufumi Hikichi
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