A two-component system plays an important role in the root-colonizing ability of Pseudomonas fluorescens strain WCS365

Molecular Plant-microbe Interactions : MPMI
L C DekkersBen Lugtenberg

Abstract

We describe the characterization of a novel Tn5lacZ colonization mutant of the efficiently colonizing Pseudomonas fluorescens strain WCS365, mutant strain PCL1210, which is at least 300- to 1,000-fold impaired in colonization of the potato root tip after co-inoculation of potato stem cuttings with a 1:1 mixture of mutant and parental cells. Similarly, the mutant is also impaired in colonization of tomato, wheat, and radish, indicating that the gene involved plays a role in the ability of P. fluorescens WCS365 to colonize a wide range of plant species. A 3.1-kb DNA fragment was found to be able to complement the observed mutation. The nucleotide sequence of the region around the Tn5lacZ insertion showed three open reading frames (ORFs). The transcriptional start site was determined. The operon is preceded by an integration host factor (IHF) binding site consensus sequence whereas no clear -10 and -35 sequences are present. The deduced amino acid sequences of the first two genes of the operon, designated as colR and colS, show strong similarity with known members of two-component regulatory systems. ColR has homology with the response regulators of the OmpR-PhoB subclass whereas ColS, the product of the gene in which the mutation...Continue Reading

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Citations

Jul 17, 2008·Comparative and Functional Genomics·C R HarwoodA Wipat
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Sandra de WeertBen J J Lugtenberg
Jul 18, 2002·Molecular Plant-microbe Interactions : MPMI·Margarita M Camacho CarvajalGuido V Bloemberg
Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Saul BurdmanPamela Ronald
Mar 6, 2013·Antimicrobial Agents and Chemotherapy·Alina D GutuSamuel M Moskowitz
Aug 29, 2006·Applied and Environmental Microbiology·Olga V MavrodiLinda S Thomashow
Sep 4, 2004·Applied and Environmental Microbiology·Manuel Espinosa-Urgel, Juan-Luis Ramos
May 6, 2004·Journal of Bacteriology·Sandra de WeertBen J J Lugtenberg
Nov 10, 2001·Annual Review of Phytopathology·B J LugtenbergG V Bloemberg
Jun 19, 2013·BMC Microbiology·Wenxiong LinRuiyu Lin
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·L C DekkersB J Lugtenberg
Oct 7, 2006·Archives of Microbiology·Daniel P RobertsJohn Lydon
Jan 14, 2005·Critical Reviews in Microbiology·E SomersM Srinivasan
Jul 1, 2009·Microbial Biotechnology·Ana SeguraJuan L Ramos
Jul 29, 2008·Environmental Microbiology·Marta PutrinsRita Hõrak
Oct 8, 2014·The Plant Pathology Journal·Young-Hee NohJae-Soon Cha
Dec 15, 2006·FEMS Microbiology Letters·Nan Hu, Bin Zhao
Feb 24, 2001·Environmental Microbiology·P B Rainey
Feb 24, 2001·Environmental Microbiology·B J Lugtenberg, L C Dekkers

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