A two-dimensional infrared study of localization, structure, and dynamics of a dipeptide in membrane environment

Biophysical Journal
V Volkov, Peter Hamm

Abstract

Using methods of time-resolved two-dimensional infrared (2D-IR) spectroscopy, we approach the problem of the structural characterization of small polypeptide systems in a membrane environment. The 2D-IR spectra recorded for a model dipeptide in different environments demonstrated a significant change in the homogeneous and inhomogeneous broadenings of the amide I resonances when the molecule inserts either into a surfactant or a phospholipid membrane. Besides the change in the diagonal features in the 2D-IR response, we observe both intramolecular and intermolecular crosspeaks between the carbonyls of the dipeptide and the phospholipid. Considering the character of the diagonal peaks and the presence of the crosspeaks, we discuss the localization of the dipeptide moieties in the membrane. Using both the anisotropy and relative intensity of the observed intramolecular crosspeaks between the two amide I modes, we provide observables that help to determine the phi/psi-dihedral angles for the backbone of the dipeptide. Time dependent studies revealed slower conformational fluctuations of the dipeptide backbone in a membrane as compared to that in an aqueous environment.

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Citations

Dec 1, 2006·The Journal of Physical Chemistry. B·Prabuddha MukherjeeMartin T Zanni
Sep 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·V V VolkovR Righini
Oct 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Haruto IshikawaMichael D Fayer
Jul 20, 2010·Annual Review of Analytical Chemistry·Michael D Fayer, Nancy E Levinger
Nov 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Chong FangRobin M Hochstrasser
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Haruto IshikawaMichael D Fayer
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Prabuddha MukherjeeMartin T Zanni
Sep 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Olaf F A LarsenSander Woutersen
Mar 28, 2006·Biophysical Journal·Casey H LonderganRobin M Hochstrasser
Mar 30, 2011·The Journal of Physical Chemistry. B·Victor V VolkovRoberto Righini
Jul 23, 2014·The Journal of Physical Chemistry. B·S LotzeH J Bakker
Mar 10, 2017·Journal of the American Chemical Society·Paul Stevenson, Andrei Tokmakoff
Feb 14, 2008·Journal of Computational Chemistry·Riccardo ChelliRoberto Righini
May 17, 2011·Physical Chemistry Chemical Physics : PCCP·Alexander PaarmannR J Dwayne Miller
Nov 21, 2009·The Journal of Physical Chemistry. B·Victor V Volkov, Roberto Righini
Feb 21, 2009·The Journal of Physical Chemistry. B·Victor V VolkovRoberto Righini
Mar 21, 2009·The Journal of Physical Chemistry. B·Sri Ram G NaraharisettyIgor V Rubtsov
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Feb 14, 2006·The Journal of Physical Chemistry. B·Victor V VolkovRoberto Righini
Feb 24, 2006·The Journal of Physical Chemistry. B·Jianping Wang, Robin M Hochstrasser
Jul 20, 2006·Journal of the American Chemical Society·Victor V VolkovRoberto Righini

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