PMID: 8612638Mar 1, 1996Paper

A two-hybrid system analysis shows interactions between 6-phosphofructo-1-kinase and 6-phosphofructo-2-kinase but not between other glycolytic enzymes of the yeast Saccharomyces cerevisiae

European Journal of Biochemistry
S MüllerF K Zimmermann

Abstract

The yeast two-hybrid system was used to investigate whether protein-protein interactions between enzymes of the early reactions of glycolysis exist in the yeast Saccharomyces cerevisiae. Various glycolytic enzymes were fused either to the GAL4 transcription-activating or DNA-binding domain and were tested for their ability to restore GAL4-dependent gene activation. All the different fusion proteins complemented the growth and enzymatic defects caused by the deletion of the respective genes, which indicates that these proteins are still functional. Interactions between the two phosphofructo-1-kinase subunits PFK1 and PFK2, interactions between the phosphofructo-2-kinase subunits, and dimerization of phosphoglucose isomerase were demonstrated. Dimerization of hexokinase 2, however, could not be demonstrated neither with N-terminal nor C-terminal fusions. A direct interaction between the hexose-6-phosphate interconverting enzymes hexokinase 2, phosphoglucose isomerase, and phosphofructo-1-kinase could also not be demonstrated. Nevertheless, our results indicate a weak interaction between phosphofructo-1-kinase and phosphofructo-2-kinase.

References

Dec 1, 1977·European Journal of Biochemistry·G KopperschlägerE Hofmann
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1979·Journal of Bacteriology·M Ciriacy, I Breitenbach
Jan 1, 1992·Current Topics in Cellular Regulation·H R Knull, J L Walsh
Aug 1, 1992·The Journal of Cell Biology·L Pagliaro, D L Taylor
May 22, 1992·Biochimica Et Biophysica Acta·C MaggettoW J Malaisse
Nov 5, 1991·Biochemistry·M Kretschmer, D G Fraenkel
Mar 5, 1991·European Journal of Pharmacology·M C Koss
Sep 2, 1991·FEBS Letters·J HeinischC P Hollenberg
Jan 1, 1991·The International Journal of Biochemistry·W J Malaisse, H Bodur
Apr 23, 1991·European Journal of Biochemistry·M KretschmerD G Fraenkel
Sep 1, 1988·The Journal of Cell Biology·L Pagliaro, D L Taylor
Apr 13, 1982·Biochemistry·D Clifton, D G Fraenkel
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Oct 15, 1981·Biochemical and Biophysical Research Communications·G Avigad
Feb 1, 1995·The International Journal of Biochemistry & Cell Biology·M al-Habori
Dec 1, 1994·Trends in Biochemical Sciences·P Srere
Aug 31, 1993·Biochemical and Biophysical Research Communications·D Carr, H Knull
Jan 1, 1993·Veterinary Research Communications·P K RoutS Naithani
Sep 1, 1993·The International Journal of Biochemistry·D Zähner, W J Malaisse
Jan 1, 1996·Health Care Management Review·K E Boles, S T Fleming

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.