A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.

PLoS Pathogens
Jennifer L KonopkaRobert E Johnston

Abstract

A concept fundamental to viral pathogenesis is that infection induces specific changes within the host cell, within specific tissues, or within the entire animal. These changes are reflected in a cascade of altered transcription patterns evident during infection. However, elucidation of this cascade in vivo has been limited by a general inability to distinguish changes occurring in the minority of infected cells from those in surrounding uninfected cells. To circumvent this inherent limitation of traditional gene expression profiling methods, an innovative mRNP-tagging technique was implemented to isolate host mRNA specifically from infected cells in vitro as well as in vivo following Venezuelan equine encephalitis virus (VEE) infection. This technique facilitated a direct characterization of the host defense response specifically within the first cells infected with VEE, while simultaneous total RNA analysis assessed the collective response of both the infected and uninfected cells. The result was a unique, multifaceted profile of the early response to VEE infection in primary dendritic cells, as well as in the draining lymph node, the initially targeted tissue in the mouse model. A dynamic environment of complex interactions ...Continue Reading

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Citations

Aug 19, 2011·Journal of Virology·Alexandra SchäferRobert E Johnston
Oct 2, 2014·Expert Review of Vaccines·Karl Ljungberg, Peter Liljeström
Sep 2, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·Leo OtsukiAndrea H Brand

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Datasets Mentioned

BETA
GeneID
16193

Methods Mentioned

BETA
PCR
co-immunoprecipitation
immunoprecipitation
immunoprecipitation assay
fluorescence-activated cell sorting
FACS
Infection
in vitro transcription
Flow Cytometry

Software Mentioned

7000 Sequence Detection
ImageQuant
Summit

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