A two-photon excited red-emissive probe for imaging mitochondria with high fidelity and its application in monitoring mitochondrial depolarization via FRET

The Analyst
Jie SunWeiying Lin

Abstract

Mitochondria play central roles in plenty of biological processes, such as apoptosis, signaling, and cell differentiation. Mitochondrial depolarization is a significant sign of deterioration of intracellular status. Although many fluorescent probes for visualizing mitochondria have been delivered, two-photon red-emitting mitochondrial probes capable of detecting mitochondrial depolarization remain rare. In this work, by linking a pyrene moiety to a quinoline salt, we have constructed two-photon red-emitting mitochondrial probes, MVQ and HVQ. Between them, HVQ with a longer side chain exhibits higher hydrophobic properties, and can image mitochondria with high-fidelity due to the aggregation caused quenching effect. In cooperation with MTDR, a commercialized mitochondrial probe, HVQ, could be used to image the depolarization of mitochondria induced by a protonophore and hydrogen peroxide. We believe that HVQ can serve as a powerful tool for the investigation of mitochondria and mitochondrial membrane potential in fundamental biological research.

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Citations

Nov 3, 2020·Chemistry : a European Journal·Kristina Ilina, Maged Henary
Dec 10, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jian YangHaijun Xu
Nov 17, 2020·Bioconjugate Chemistry·Cindy MaShana O Kelley

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Methods Mentioned

BETA
imaging technique
FRET
electrophoresis
fluorescence imaging
Fluorescence

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis