A two-site immunofluorometric assay for human placental lactogen

Clinica Chimica Acta; International Journal of Clinical Chemistry
L ViinikkaM Pourfarzaneh

Abstract

A two-site immunofluorometric assay for human placental lactogen (HPL) in serum has been developed. Samples and standards are incubated for 10 min with an excess of sheep anti-HPL serum covalently coupled to particles of magnetisable cellulose. After sedimenting the particles and adsorbed hormone on a magnet the supernates are aspirated (or decanted) to waste. An excess of purified sheep anti-HPL immunoglobulin, labelled with fluorescein, is added and, after a further 20 min, the fluorescence remaining in the supernates, after sedimenting the particles, is inversely related to the initial concentration of HPL. Results correlate closely with those of an established radioimmunoassay (r = 0.92), within- and between-assay coefficients of variation are less than 10% and, employing a 200 microliter sample volume, the assay extends from a minimum detection limit of 0.02 mg/l throughout the entire range of values encountered in pregnancy to more than 10 mg/l.

References

Jan 1, 1976·Journal of Clinical Pathology. Supplement (Royal College of Pathologists)·T Chard
Nov 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·R D NargessiD S Smith
Feb 1, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·H van HellA H Schuurs
Oct 12, 1974·Lancet·T Chard
Sep 30, 1972·British Medical Journal·P A NivenT Chard
Jun 1, 1971·The Journal of Obstetrics and Gynaecology of the British Commonwealth·A T LetchworthT Chard

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