PMID: 9175267Jan 1, 1996Paper

A tyrosine kinase-like molecule is localized in the nuclear membrane of neurons: hippocampal behavior under stress

Biology of the Cell
Y KajiiK Onodera

Abstract

Protein tyrosine kinases play important roles in the development of the mammalian nervous system during embryogenesis and in the maintenance of function of the adult brain. Using a semi-nested PCR technique based on a short amino acid motif of protein tyrosine kinases, we isolated a human genomic DNA encoding a peptide whose sequence was related to known mammalian protein tyrosine kinases. The expression was examined by Northern blot analysis, and transcripts were detected almost exclusively in the brain. The corresponding cDNA was sequenced, and it was revealed that the gene designated as byk coded for a receptor-like molecule with a motif of protein tyrosine kinase. Immunohistochemical analysis demonstrated that the Byk protein was expressed in neurons and was located in the nuclear envelope. To understand the physiological significance of the Byk protein, we investigated the behavior of this molecule in the hippocampus after ischemia. Byk-like immunoreactivity disappeared from the neurons in the fields CA1 through CA3 and the dentate gyrus of the hippocampus following 20 min of ischemia. After recirculation of blood flow, neurons in the CA3 field and the dentate gyrus re-expressed Byk-like antigen but CA1 neurons did not. In...Continue Reading

References

May 1, 1979·Stroke; a Journal of Cerebral Circulation·W A Pulsinelli, J B Brierley
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Apr 29, 1991·Neuroscience Letters·K AbeK Kogure
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A F Wilks
Apr 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·B K Siesjö, F Bengtsson
Jun 1, 1989·Neuron·Y A Barde
Sep 11, 1987·Cell·T Hunter
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
May 1, 1994·Trends in Neurosciences·R M LindsayP S DiStefano
Feb 16, 1995·Nature·T Pawson
Dec 1, 1993·Stroke; a Journal of Cerebral Circulation·K Kogure, H Kato

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