A tyrosine-phosphorylated 12-amino-acid sequence of enteropathogenic Escherichia coli Tir binds the host adaptor protein Nck and is required for Nck localization to actin pedestals

Molecular Microbiology
Kenneth G CampelloneJohn M Leong

Abstract

Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) each promote the reorganization of actin into filamentous pedestal structures beneath attached bacteria during colonization of the intestinal epithelium. Central to this process is the translocation of the protein Tir (translocated intimin receptor) into the plasma membrane of host cells, where it interacts with the bacterial outer membrane protein intimin and triggers cellular signalling events that lead to actin rearrangement. Actin signalling by EPEC Tir requires a tyrosine residue, Y474, which is phosphorylated in the host cell. In contrast, EHEC Tir lacks this residue and generates pedestals independently of tyrosine phosphorylation. Consistent with this difference, recent work indicates that EHEC Tir cannot functionally replace EPEC Tir. To identify the role that tyrosine phosphorylation of EPEC Tir plays in actin signalling, we generated chimeric EHEC/EPEC Tir proteins and identified a 12-residue sequence of EPEC Tir containing Y474 that confers actin-signalling capabilities to EHEC Tir when the chimera is expressed in EPEC. Nck, a mammalian adaptor protein that has been implicated in the initiation of actin signalling, binds to this sequence ...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A E JerseJ B Kaper
Nov 1, 1990·Virology·S J GoebelE Paoletti
Mar 24, 1983·The New England Journal of Medicine·L W RileyM L Cohen
Oct 1, 1995·Molecular and Cellular Biology·O M Rivero-LezcanoK C Robbins
Sep 1, 1993·The Journal of Clinical Investigation·M S DonnenbergM M Levine
Nov 1, 1995·Trends in Biochemical Sciences·Z Songyang, L C Cantley
Feb 12, 1998·Science·T Pawson, J D Scott
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·N RameshR S Geha
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Aug 8, 1998·The Journal of Biological Chemistry·I M AntónR S Geha
Jan 1, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J H McCarty
Apr 27, 1999·Molecular Microbiology·E L HartlandG Frankel
May 29, 1999·Journal of Bacteriology·F BeltramettiC A Guzmán
Apr 18, 2000·Gene·K C MurphyA R Poteete
Jan 9, 2001·Trends in Cell Biology·F Frischknecht, M Way
May 31, 2001·Science·D PantaloniM F Carlier
Feb 28, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M. VargasJ. Vila

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Citations

Mar 5, 2003·Current Opinion in Microbiology·Kenneth G Campellone, John M Leong
Jan 18, 2006·Nature Reviews. Microbiology·Joanne M StevensMark P Stevens
Apr 4, 2006·Nature Reviews. Microbiology·Richard D HaywardKenneth G Campellone
Apr 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Didier VingadassalomJohn M Leong
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Fatemeh SafariMasanori Hatakeyama
Nov 19, 2008·Antimicrobial Agents and Chemotherapy·Ning J PanJon D Goguen
Jul 15, 2003·Clinical Microbiology Reviews·S C ClarkeP H Williams
Jan 24, 2006·Infection and Immunity·Wensheng Luo, Michael S Donnenberg
Jan 24, 2008·Molecular and Cellular Biology·Ivan M BlasutigTony Pawson
Oct 9, 2012·The Journal of Clinical Investigation·Emily M MallickDavid B Schauer
Dec 16, 2003·BMC Molecular Biology·Kenan C Murphy, Kenneth G Campellone
Jun 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·D R CliftonT Hackstadt
Mar 20, 2013·BioMed Research International·Fernando Navarro-GarciaLucia Chavez-Dueñas
Dec 17, 2014·BioMed Research International·Narcisa Martinez-QuilesPhilip R Hardwidge
Feb 6, 2004·The Journal of Cell Biology·Kenneth G CampelloneJohn M Leong
Nov 23, 2011·The Journal of Physiology·Kim Hodges, Gail Hecht
Sep 10, 2013·Infectious Disease Clinics of North America·Dakshina M JandhyalaJohn M Leong
Mar 19, 2005·Molecular Microbiology·Mark P StevensEdouard E Galyov
Jun 5, 2003·Cellular Microbiology·Jean-Philippe NougayrèdeMichael S Donnenberg

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