A tyrosine-phosphorylated 55-kilodalton motility-associated bovine sperm protein is regulated by cyclic adenosine 3',5'-monophosphates and calcium

Biology of Reproduction
S VijayaraghavanD W Carr

Abstract

Sperm motility is regulated by protein phosphorylation. We have recently shown that a serine/threonine phosphatase system is involved in motility regulation. Two of the components of the phosphatase system, GSK-3 and PP1gamma2, are regulated by tyrosine phosphorylation. During our investigation of sperm tyrosine-phosphorylated proteins we discovered a 55-kDa protein whose tyrosine phosphorylation correlates closely to the motility state of sperm. This protein is tyrosine phosphorylated to a much higher degree in motile caudal than in immotile caput epididymal sperm. Motility inhibition of caudal epididymal sperm by protein kinase A (PKA) anchoring inhibition or by ionomycin-induced calcium overload led to the virtual disappearance of tyrosine phosphorylation of the 55-kDa protein. Conversely, treatment of sperm with motility activators, isobutylmethylxanthine or 8-bromo-cAMP, resulted in increased tyrosine phosphorylation of the protein. The protein was present in the soluble 100 000 x g supernatants of sperm extracts and was heat labile. Chromatography through diethylaminoethyl-cellulose and Western blot analysis showed that this 55-kDa protein is not a regulatory subunit of PKA or alpha-tubulin. Our results represent the iden...Continue Reading

Citations

Jan 28, 1998·Molecular Reproduction and Development·K AshizawaY Tsuzuki
Apr 25, 2000·Biochemical and Biophysical Research Communications·O J D'CruzF M Uckun
Feb 16, 2006·Reproduction, Fertility, and Development·Regina M Turner
Mar 24, 2011·Reproduction, Fertility, and Development·J M Fernández-NovellJ E Rodríguez-Gil
Dec 2, 2011·Molecular Biology of the Cell·Xianlong GaoTarun B Patel
Aug 2, 2003·Biology of Reproduction·Heath EcroydR John Aitken
Nov 13, 2004·Reproductive Biology and Endocrinology : RB&E·Rajesh K Naz, Preeti B Rajesh
Aug 22, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·I M AparicioL J Garcia-Marin
Mar 19, 2002·Human Fertility : Journal of the British Fertility Society·W Chris L Ford
Nov 26, 2003·International Journal of Andrology·K M Williams, W C L Ford
Aug 7, 2010·Bioelectromagnetics·Roberto IorioRosella Cardigno Colonna
Nov 28, 2006·Biochemical and Biophysical Research Communications·Visesato MorTapati Chatterjee
Oct 22, 2016·Cytoskeleton·Charles B Lindemann, Kathleen A Lesich
May 18, 2017·Molecular Reproduction and Development·Luis ÁguilaRicardo Felmer
Aug 31, 2001·Human Reproduction·M LuconiE Baldi
Jan 9, 2015·Biology of Reproduction·Rahul BhattacharjeeSrinivasan Vijayaraghavan
Dec 3, 2004·Reproductive Medicine and Biology·Masakatsu FujinokiHideki Ohtake
May 12, 2006·Biology of Reproduction·Jacob C ThundathilMary M Buhr
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard O Williams
Oct 29, 2003·Journal of Andrology·Regina M Turner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.