PMID: 7535919Nov 1, 1994Paper

A ubiquitous non-selective cation channel in the mouse renal tubule with variable sensitivity to calcium

Pflügers Archiv : European journal of physiology
A ChraïbiJacques Teulon

Abstract

Basolateral membranes of microdissected collagenase-treated fragments of renal tubules from the mouse were examined using the cell-attached and the cell-free variants of the patch-clamp technique. With a K(+)-rich solution in the pipette, a highly active, inwardly rectifying K+ channel was observed on intact cells of the cortical collecting tubule (CCT). The mean inward and outward conductances were 38.5 +/- 3.1 pS and 17.3 +/- 1.8 pS, respectively (n = 4). In contrast, cell-attached patches were usually inactive when a Na(+)-rich solution filled the patch pipette. However, another type of channel with a conductance of 20-30 pS exhibited a sparse activity in 4/20 CCT. In excised, inside-out patches, the most frequent channel in CCT had an ohmic unit conductance of 27.1 +/- 1.2 pS (n = 17), excluded anions (PCl/PNa = 0.09), discriminated little between NH4+, K+ and Na+ (PNH4/PNa = 1.5; PK/PNa = 0.9), and was much less permeable to Ca2+ and Ba2+ than to Na+ (PCa/PNa = 0.09; PBa/PNa approximately 0). The cation channel was moderately voltage-dependent, showing a decreased open probability (Po) at negative voltages. It was activated by internal calcium (threshold: 1 mumol/l-0.1 mmol/l calcium), and inhibited by the adenine nucleoti...Continue Reading

References

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Citations

Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·T VolkC Korbmacher
Apr 2, 2003·The Journal of General Physiology·Stéphane LourdelJacques Teulon
Jan 18, 2014·British Journal of Pharmacology·R GuinamardC A Del Negro
Jan 11, 2012·Biochimica Et Biophysica Acta·Romain GuinamardJacques Teulon
Jun 17, 2010·Physiology·Romain GuinamardPierre Launay
May 21, 2014·Journal of Neuroscience Research·Mayumi SakurabaKatsuhisa Ikeda
Apr 24, 2003·American Journal of Physiology. Renal Physiology·Jean-Yves LapointeYasunobu Okada
Jan 13, 2006·American Journal of Physiology. Renal Physiology·Antoine NissantJacques Teulon

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