A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is essential for viability in Drosophila melanogaster

The Journal of Biological Chemistry
Kelly G Ten Hagen, Duy T Tran

Abstract

We report the first demonstration that the activity of a member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase gene family is necessary for viability in Drosophila melanogaster. Expression of the wild-type recombinant pgant35A gene in COS7 cells resulted in in vitro activity against peptide and glycopeptide substrates, demonstrating that this gene encodes a biochemically active transferase. Previous mutagenesis studies identified recessive lethal mutations that were rescued by a genomic fragment containing the pgant35A gene; however, the presence of additional open reading frames within this fragment left open the possibility that another gene was responsible for rescue of the observed lethality. Here, we have determined the molecular nature of the mutations in three independent mutant alleles. Two of the mutant alleles contain premature stop codons within the coding region of pgant35A. The third mutant contains an arginine to tryptophan amino acid change, which, when expressed in COS7 cells, resulted in a dramatic reduction of transferase activity in vitro. PCR amplification of this gene from Drosophila cDNA panels and Northern analysis revealed that it is expressed throughout embryonic, larval, and pupal stag...Continue Reading

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Citations

Aug 13, 2008·Glycoconjugate Journal·E Tian, Kelly G Ten Hagen
Mar 9, 2004·International Journal for Parasitology·Magdalena M Stwora-WojczykBoguslaw S Wojczyk
Sep 27, 2003·Molecular and Biochemical Parasitology·Boguslaw S WojczykSteven L Spitalnik
Dec 5, 2002·Current Opinion in Structural Biology·Iain B H Wilson
Sep 3, 2010·The Journal of Biological Chemistry·Liping Zhang, Kelly G Ten Hagen
Oct 7, 2008·The Journal of Biological Chemistry·Liping ZhangKelly G Ten Hagen
Jan 19, 2013·The Journal of Biological Chemistry·Duy T Tran, Kelly G Ten Hagen
Oct 1, 2008·Glycobiology·Kelly G ten HagenYing Zhang
Jun 11, 2004·Annual Review of Biochemistry·Robert S Haltiwanger, John B Lowe
Oct 7, 2004·Biological & Pharmaceutical Bulletin·Naosuke NakamuraAkira Kurosaka
Oct 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Timothy A FritzLawrence A Tabak
Jun 13, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erika Staudacher
Mar 13, 2016·Biochimica Et Biophysica Acta·Joanne ChiaFrederic Bard
Jan 27, 2011·Biochemical Society Transactions·Liping Zhang, Kelly G Ten Hagen
May 5, 2004·Chemistry & Biology·Howard C HangCarolyn R Bertozzi
Jul 24, 2004·Chemistry & Biology·Nicola Pohl
Jul 12, 2005·Bioorganic & Medicinal Chemistry·Howard C Hang, Carolyn R Bertozzi
Sep 26, 2007·International Journal for Parasitology·Andrea MedeirosEduardo Osinaga
Nov 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yuh-Ru LinKenneth D Irvine
Nov 14, 2006·The Journal of Biological Chemistry·E Tian, Kelly G Ten Hagen
May 2, 2019·Molecular & Cellular Proteomics : MCP·Yoshiki NarimatsuSergey Y Vakhrushev

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