A unified approach to molecular epidemiology investigations: tools and patterns in California as a case study for endemic shigellosis.

BMC Infectious Diseases
Sawsan Al-NimriLily Chen

Abstract

Shigellosis causes diarrheal disease in humans from both developed and developing countries, and multi-drug resistance is an emerging problem. The objective of this study is to present a unified approach that can be used to characterize endemic and outbreak patterns of shigellosis using use a suite of epidemiologic and molecular techniques. The approach is applied to a California case study example of endemic shigellosis at the population level. Epidemiologic patterns were evaluated with respect to demographics, multi-drug resistance, antimicrobial resistance genes, plasmid profiles, and pulsed-field gel electrophoresis (PFGE) fingerprints for the 43 Shigella isolates obtained by the Monterey region health departments over the two year period from 2004-2005. The traditional epidemiologic as well as molecular epidemiologic findings were consistent with endemic as compared to outbreak shigellosis in this population. A steady low level of cases was observed throughout the study period and high diversity was observed among strains. In contrast to most studies in developed countries, the predominant species was Shigella flexneri (51%) followed closely by S. sonnei (49%). Over 95% of Shigella isolates were fully resistant to three or...Continue Reading

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Apr 18, 2012·Foodborne Pathogens and Disease·Mariana PichelUNKNOWN Shigella flexneri PulseNet PFGE Protocol Working Group
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Jul 11, 2019·Annals of Clinical Microbiology and Antimicrobials·Siraj HussenZemenu Yohannes Kassa

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Stata
BioNumerics

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