A unigene catalogue of 5700 expressed genes in cassava

Plant Molecular Biology
C LopezV Verdier

Abstract

Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv.manihotis. We report here the single pass sequencing of 11,954 cDNA clones from the 5' ends, including 111 from the 3' ends. Cluster analysis permitted the identification of a unigene set of 5,700 sequences. Sequence analyses permitted the assignment of a putative functional category for 37% of sequences whereas approximately 16% sequences did not show any significant similarity with other proteins present in the database and therefore can be considered as cassava specific genes. A group of genes belonging to a large multigene family was identified. We characterize a set of genes detected only in infected libraries putatively involved in the defense response to pathogen infection. By comparing two libraries obtained from cultivars contrasting in their starch content a group of genes associated to starch biosynthesis and differentially expressed was identified. This is...Continue Reading

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Citations

Jan 15, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C LopezV Verdier
Jan 5, 2005·Plant Molecular Biology·Ismael B F FofanaClaude M Fauquet
Dec 7, 2007·Genome Génome / Conseil National De Recherches Canada·Camilo E LópezValérie Verdier
Jun 15, 2010·BMC Microbiology·Mauricio Soto-SuárezValérie Verdier
Nov 10, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Morag E FergusonEtienne P de Villiers
Jun 30, 2007·Journal of Molecular and Cellular Cardiology·Inju ParkChunghee Cho
May 1, 2008·Molecular Ecology Resources·S TangphatsornruangK Triwitayakorn
Jun 22, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hernán CeballosClair H Hershey
Aug 20, 2016·Scientific Reports·Zehong DingPinghua Li
Feb 20, 2016·Scientific Reports·You-Zhi LiBao-Shan Chen
Jun 15, 2007·Annals of Botany·Abelmon S GesteiraJúlio C M Cascardo
Jun 8, 2012·Molecular Plant Pathology·John MansfieldGary D Foster
Jan 3, 2006·Plant Biology·R MatyssekD Treutter
Jul 7, 2021·Molecular Plant Pathology·Carlos A Zárate-ChavesBoris Szurek

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