A unique CE16 acetyl esterase from Podospora anserina active on polymeric xylan

Applied Microbiology and Biotechnology
Vladimír PuchartPeter Biely

Abstract

The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed in Pichia pastoris and purified to electrophoretic homogeneity. The P. anserina CE16 enzyme (PaCE16A) exhibited different catalytic properties than so far known CE16 esterases represented by the Trichoderma reesei CE16 acetyl esterase (TrCE16). A common property of both CE16 esterases is their exodeacetylase activity, i.e., deesterification at positions 3 and 4 of monomeric xylosides and the nonreducing end xylopyranosyl (Xylp) residue of oligomeric homologues. However, the PaCE16A showed lower positional specificity than TrCE16 and efficiently deacetylated also position 2. The major difference observed between PaCE16A and TrCE16 was found on polymeric substrate, acetylglucuronoxylan. While TrCE16 does not attack internal acetyl groups, PaCE16A deacetylated singly and doubly acetylated Xylp residues in the polymer to such an extent that it resulted in the polymer precipitation. Similarly as typical acetylxylan este...Continue Reading

References

May 8, 2008·Genome Biology·Eric EspagnePhilippe Silar
Nov 4, 2008·Applied and Environmental Microbiology·Xin-Liang LiPeter Biely
Feb 5, 2013·Biochimica Et Biophysica Acta·Iveta UhliarikováPeter Biely
Oct 17, 2013·Biochimica Et Biophysica Acta·Peter BielyBjorge Westereng
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat
Apr 4, 2014·Applied Microbiology and Biotechnology·Laetitia PoidevinEric Record
Nov 9, 2014·Applied Microbiology and Biotechnology·Vladimír Puchart, Peter Biely

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Citations

Jul 21, 2016·Journal of Biotechnology·Vladimír PuchartPeter Biely
Oct 6, 2016·Enzyme and Microbial Technology·Fiyinfoluwa A AdesioyeDon A Cowan
Mar 29, 2018·Biotechnology for Biofuels·Fakhria M RazeqEmma R Master

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