PMID: 2910878Jan 1, 1989Paper

A unique cytoskeleton associated with crawling in the amoeboid sperm of the nematode, Ascaris suum

The Journal of Cell Biology
S SepsenwolT M Roberts

Abstract

Nematode sperm extend pseudopods and pull themselves over substrates. They lack an axoneme or the actin and myosins of other types of motile cells, but their pseudopods contain abundant major sperm protein (MSP), a family of 14-kD polypeptides found exclusively in male gametes. Using high voltage electron microscopy, a unique cytoskeleton was discovered in the pseudopod of in vitro-activated, crawling sperm of the pig intestinal nematode Ascaris suum. It consists of 5-10-nm fuzzy fibers organized into 150-250-nm-thick fiber complexes, which connect to each of the moving pseudopodial membrane projections, villipodia, which in turn make contact with the substrate. Individual fibers in a complex splay out radially from its axis in all directions. The centripetal ends intercalate with fibers from other complexes or terminate in a thickened layer just beneath the pseudopod membrane. Monoclonal antibodies directed against MSP heavily label the fiber complexes as well as individual pseudopodial filaments throughout their length. This represents the first evidence that MSP may be the major filament protein in the Ascaris sperm cytoskeleton. The large fiber complexes can be seen clearly in the pseudopods of live, crawling sperm by compu...Continue Reading

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Citations

Nov 1, 1996·Molecular Reproduction and Development·A Mansir, J L Justine
Jan 1, 1990·Cell Motility and the Cytoskeleton·S Sepsenwol, S J Taft
Sep 1, 1989·Molecular and Biochemical Parasitology·A L ScottS Ward
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Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ellen L BatchelderJulie Plastino
May 19, 2010·BMC Developmental Biology·Peter HegerEinhard Schierenberg
May 10, 2012·PLoS Genetics·Madhura KulkarniHarold E Smith
Sep 10, 2013·Journal of Experimental Botany·Amir Sanati Nezhad, Anja Geitmann
Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Long MiaoThomas M Roberts
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Dec 22, 2006·Cell Motility and the Cytoskeleton·Long MiaoThomas M Roberts
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