PMID: 9541534Apr 29, 1998Paper

A unique fungal lysine biosynthesis enzyme shares a common ancestor with tricarboxylic acid cycle and leucine biosynthetic enzymes found in diverse organisms

Journal of Molecular Evolution
S D Irvin, J K Bhattacharjee

Abstract

Fungi have evolved a unique alpha-amino-adipate pathway for lysine biosynthesis. The fungal-specific enzyme homoaconitate hydratase from this pathway is moderately similar to the aconitase-family proteins from a diverse array of taxonomic groups, which have varying modes of obtaining lysine. We have used the similarity of homoaconitate hydratase to isopropylmalate isomerase (serving in leucine biosynthesis), aconitase (from the tricarboxylic acid cycle), and iron-responsive element binding proteins (cytosolic aconitase) from fungi and other eukaryotes, eubacteria, and archaea to evaluate possible evolutionary scenarios for the origin of this pathway. Refined sequence alignments show that aconitase active site residues are highly conserved in each of the enzymes, and intervening sequence sites are quite dissimilar. This pattern suggests strong purifying selection has acted to preserve the aconitase active site residues for a common catalytic mechanism; numerous other substitutions occur due to adaptive evolution or simply lack of functional constraint. We hypothesize that the similarities are the remnants of an ancestral gene duplication, which may not have occurred within the fungal lineage. Maximum likelihood, neighbor joining...Continue Reading

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Citations

Feb 20, 2002·FEBS Letters·Junichi MiyazakiHisakazu Yamane
Apr 27, 2010·Applied and Environmental Microbiology·Zakee L SabreeNancy A Moran
May 11, 2000·Molecular and Cellular Biology·L T Jensen, V C Culotta
Aug 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ashraf AlhapelAntonio J Pierik
Sep 10, 2003·Journal of Molecular Biology·Hiroaki SakaiShigeyuki Yokoyama
Jul 4, 2002·FEBS Letters·Asri Peni WulandariHisakazu Yamane
May 7, 2011·Natural Product Reports·Tohru DairiIsao Fujii
Aug 28, 2016·The Journal of Biological Chemistry·Ayako YoshidaMakoto Nishiyama
Nov 21, 2021·Nature Metabolism·Jakob VowinckelMarkus Ralser

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