A unique, highly conserved secretory invertase is differentially expressed by promastigote developmental forms of all species of the human pathogen, Leishmania

Molecular and Cellular Biochemistry
Todd A LydaD M Dwyer

Abstract

Leishmania are protozoan pathogens of humans that exist as extracellular promastigotes in the gut of their sand fly vectors and as obligate intracellular amastigotes within phagolysosomes of infected macrophages. Between infectious blood meal feeds, sand flies take plant juice meals that contain sucrose and store these sugars in their crop. Such sugars are regurgitated into the sand fly anterior midgut where they impact the developing promastigote parasite population. In this report we showed that promastigotes of all Leishmania species secreted an invertase/sucrase enzyme during their growth in vitro. In contrast, neither L. donovani nor L. mexicana amastigotes possessed any detectable invertase activity. Importantly, no released/secreted invertase activity was detected in culture supernatants from either Trypanosoma brucei or Trypanosoma cruzi. Using HPLC, the L. donovani secretory invertase was isolated and subjected to amino acid sequencing. Subsequently, we used a molecular approach to identify the LdINV and LmexINV genes encoding the ~72 kDa invertases produced by these organisms. Interestingly, we identified high fidelity LdINV-like homologs in the genomes of all Leishmania sp. but none were present in either T. brucei o...Continue Reading

References

Jun 29, 1990·Cell·N Agabian
Jan 1, 1986·Annual Review of Biochemistry·P Borst
Oct 1, 1988·Molecular and Biochemical Parasitology·D Fong, B Lee
Dec 1, 1987·Molecular and Biochemical Parasitology·P A Bates, D M Dwyer
Jul 1, 1984·Molecular and Biochemical Parasitology·D Zilberstein, D M Dwyer
Mar 30, 1984·Science·D L Sacks, P V Perkins
Aug 1, 1994·Molecular and Biochemical Parasitology·O FernandesD A Campbell
May 1, 1994·The Journal of Eukaryotic Microbiology·J J Blum, F R Opperdoes
Jul 1, 1993·Clinical Microbiology Reviews·G Grimaldi, R B Tesh
Jan 1, 1996·Annual Review of Cell and Developmental Biology·R D Teasdale, M R Jackson
Aug 29, 1998·The Journal of Biological Chemistry·E GhedinG Matlashewski
Jan 8, 1999·Molecular and Biochemical Parasitology·C ClaytonL Vanhamme
Mar 15, 2000·Annals of Tropical Medicine and Parasitology·J K StilesJ C Meade
Apr 9, 2001·Clinical Microbiology Reviews·E Handman
Sep 7, 2001·Annual Review of Microbiology·D Sacks, S Kamhawi
Sep 6, 2002·Insect Biochemistry and Molecular Biology·J G ValenzuelaJ M C Ribeiro
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard J S BurchmoreScott M Landfear
Jun 28, 2003·The Journal of Biological Chemistry·Wen-Wei ZhangGreg Matlashewski
Mar 24, 2004·International Journal for Parasitology·Alain DebrabantDennis M Dwyer
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Sep 11, 2004·Current Molecular Medicine·P A Bates, M E Rogers
Jul 18, 2006·Trends in Parasitology·Shaden Kamhawi
Feb 6, 2007·The Journal of Biological Chemistry·Manju B Joshi, Dennis M Dwyer
Nov 3, 2010·Cellular Microbiology·Judith Maxwell Silverman, Neil E Reiner
Apr 26, 2011·International Journal for Parasitology·Arpita Singh, Debjani Mandal
Apr 3, 2012·Journal of Cell Science·Khoa D TranScott M Landfear
Jan 1, 2011·Frontiers in Cellular and Infection Microbiology·Judith Maxwell Silverman, Neil E Reiner
Nov 13, 2013·Nucleic Acids Research·Dennis A BensonEric W Sayers
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta

❮ Previous
Next ❯

Citations

May 18, 2016·Biochemistry Research International·Arpita Singh, Debjani Mandal
May 11, 2016·PLoS Neglected Tropical Diseases·Pedro J AlcoleaVicente Larraga
May 23, 2019·Memórias do Instituto Oswaldo Cruz·Samara G da CostaFernando Ariel Genta

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.