A unique in vivo experimental approach reveals metabolic adaptation of the probiotic Propionibacterium freudenreichii to the colon environment

BMC Genomics
Taous SaraouiHélène Falentin

Abstract

Propionibacterium freudenreichii is a food grade bacterium consumed both in cheeses and in probiotic preparations. Its promising probiotic potential, relying largely on the active release of beneficial metabolites within the gut as well as the expression of key surface proteins involved in immunomodulation, deserves to be explored more deeply. Adaptation to the colon environment is requisite for the active release of propionibacterial beneficial metabolites and constitutes a bottleneck for metabolic activity in vivo. Mechanisms allowing P. freudenreichii to adapt to digestive stresses have been only studied in vitro so far. Our aim was therefore to study P. freudenreichii metabolic adaptation to intra-colonic conditions in situ. We maintained a pure culture of the type strain P. freudenreichii CIRM BIA 1, contained in a dialysis bag, within the colon of vigilant piglets during 24 hours. A transcriptomic analysis compared gene expression to identify the metabolic pathways induced by this environment, versus control cultures maintained in spent culture medium.We observed drastic changes in the catabolism of sugars and amino-acids. Glycolysis, the Wood-Werkman cycle and the oxidative phosphorylation pathways were down-regulated bu...Continue Reading

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Citations

May 14, 2016·Nutrition Research Reviews·Eugeni RouraDavid Val-Laillet
May 16, 2017·Microorganisms·Houem RabahGwénaël Jan
Feb 12, 2017·Applied Microbiology and Biotechnology·Mickaël Alain FleuryStéphanie Blanquet-Diot
Dec 10, 2016·BMC Genomics·Flavia Figueira AburjaileHélène Falentin
Aug 2, 2020·Applied and Environmental Microbiology·Keita NishiyamaNobuhiko Okada

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Datasets Mentioned

BETA
GSE50089

Methods Mentioned

BETA
sedation
PCR
Feature Extraction
reverse transcription quantitative PCR
protein folding

Software Mentioned

Excel
Agilent Feature Extraction
limma
Primer3 Plus
geNorm VBA
geNorm
R

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