Oct 18, 2007

A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development

Genesis : the Journal of Genetics and Development
Yu LanRulang Jiang


Mammalian palate development is a multistep process, involving initial bilateral downward outgrowth of the palatal shelves from the oral side of the maxillary processes, followed by stage-specific palatal shelf elevation to the horizontal position above the developing tongue and subsequent fusion of the bilateral palatal shelves at the midline to form the intact roof of the oral cavity. While mutations in many genes have been associated with cleft palate pathogenesis, the molecular mechanisms regulating palatal shelf growth, patterning, and elevation are not well understood. Genetic studies of the molecular mechanisms controlling palate development in mutant mouse models are often complicated by early embryonic lethality or gross craniofacial malformation. We report here the development of a mouse strain for tissue-specific analysis of gene function in palate development. We inserted an IresCre bicistronic expression cassette into the 3' untranslated region of the mouse Osr2 gene through gene targeting. We show, upon crossing to the R26R reporter mice, that Cre expression from the Osr2(IresCre) knockin allele activated beta-galactosidase expression specifically throughout the developing palatal mesenchyme from the onset of pala...Continue Reading

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Mentioned in this Paper

Pathogenic Aspects
Entire Oral Cavity
Cre recombinase, Enterobacteria phage P1
OSR2 gene
Structure of Papilla Incisiva of Mouth
Cleft Palate, Isolated
Recombination, Genetic

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