A unique neuroendocrine cell model derived from the human foetal neural crest.

Journal of Endocrinological Investigation
E RapizziA Peri

Abstract

Nowadays, no human neuroendocrine cell models derived from the neural crest are available. In this study, we present non-transformed long-term primary Neural Crest Cells (NCCs) isolated from the trunk region of the neural crest at VIII-XII gestational weeks of human foetuses obtained from voluntary legal abortion. In NCC, quantitative real-time RT PCR demonstrated the expression of neural crest specifier genes, such as Snail1, Snail2/SLUG, Sox10, FoxD3, c-Myc, and p75NTR. Moreover, these cell populations expressed stemness markers (such as Nanog and nestin), as well as markers of motility and invasion (TAGLN, MMP9, CXCR4, and CXCR7), and of neuronal/glial differentiation (MAP2, GFAP, SYP, and TAU). Functional analysis demonstrated that these cells not only possessed high migration properties, but most importantly, they expressed markers of sympatho-adrenal lineage, such as ASCL1 and tyrosine hydroxylase (TH). Moreover, the expression of TH increased after the induction with two different protocols of differentiation towards neuronal and sympatho-adrenal phenotypes. Finally, exposure to conditioned culture media from NCC induced a mature phenotype in a neuronal cell model (namely SH-SY5Y), suggesting that NCC may also act like S...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Dec 1, 1990·Current Opinion in Cell Biology·J A Wagner, S K Kostyk
Jul 1, 1988·Trends in Neurosciences·E M JohnsonP S DiStefano
Jan 1, 1986·Current Topics in Developmental Biology·M OgawaH Ohta
May 1, 1987·Developmental Biology·J F Loring, C A Erickson
Dec 1, 1994·Journal of Neurology·R P Bunge
Oct 1, 1993·Current Opinion in Neurobiology·M L Reynolds, C J Woolf
Feb 1, 1993·Journal of Neurobiology·M Bronner-Fraser
Jul 6, 2000·Brain Research. Developmental Brain Research·Y ChengP J Scotting
Jan 11, 2001·Cell and Tissue Research·J F PowersA S Tischler
Jul 26, 2005·Seminars in Cell & Developmental Biology·Meyer Barembaum, Marianne Bronner-Fraser
Aug 24, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Robert N Kelsh
Oct 14, 2006·Stem Cells·Tsutomu MotohashiTakahiro Kunisada
Sep 13, 2007·Journal of Anatomy·Ronan O'Rahilly, Fabiola Müller
Feb 5, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Haiying YuOliver Eickelberg
Jul 8, 2008·Experimental Neurology·Pasquale GallinaNicola Di Lorenzo
Sep 6, 2008·Neuroendocrinology·Linda CsöreghGabriel Fried
Jan 27, 2009·Clinical & Experimental Metastasis·Lucia MartiniovaKarel Pacak
Mar 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Quenten SchwarzChristiana Ruhrberg
May 19, 2010·Developmental Biology·Erin BettersMartín I García-Castro
Jul 21, 2010·Developmental Cell·Eric TheveneauRoberto Mayor
Feb 22, 2012·Developmental Biology·Efrat Monsonego-OrnanDalit Sela-Donenfeld
Mar 31, 2012·Developmental Biology·Marianne E Bronner, Carole LaBonne
Jan 5, 2013·Nature Protocols·Laura MenendezStephen Dalton
Jun 21, 2013·PloS One·Hans K GhayeeJerry W Shay
Nov 11, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lillias H MaguireAllan M Goldstein
Nov 12, 2014·Nature Reviews. Endocrinology·Judith FavierAnne-Paule Gimenez-Roqueplo
Mar 27, 2015·Endocrine-related Cancer·Elena RapizziMassimo Mannelli
Apr 24, 2015·Nature·Stephen A GreenMarianne E Bronner
Jan 28, 2016·Scientific Reports·Miller HuangWilliam A Weiss
Jan 29, 2016·Seminars in Cell & Developmental Biology·Adam Shellard, Roberto Mayor
Oct 7, 2016·Endocrine Pathology·James F PowersArthur S Tischler
Sep 25, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giada PoliMichaela Luconi
Dec 13, 2018·Frontiers in Endocrinology·Rami AlrezkKarel Pacak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.