A unique population of CD34+ cells in cord blood

Stem Cells
M T NimgaonkarE D Ball

Abstract

Human umbilical cord blood (CB) is a rich source of hematopoietic stem cells for both research and stem cell transplantation. In clinical studies, it appears that recovery from myeloablative therapy using CB requires significantly fewer cells than a typical allogeneic marrow transplant. This suggests that CB may be enriched for early hematopoietic progenitors. The present studies were undertaken to determine the presence of CD34+ cells in CB with the phenotypic characteristics of multipotential stem cells. In 22 CB harvests, the average percentage of CD34+ cells was 1.33 +/- 0.21% (SE), a value similar to that in adult normal bone marrows (BM). However, the distribution of CD34+ cells was distinctly different from either BM or granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood stem cell harvests. CB contained a defined population of brightly staining CD34+ cells with low side scatter. These CD34 (bright) cells comprised a mean of 14.5 +/- 2.5% of the CB CD34+ cells, whereas < 1% of BM CD34+ cells has been shown to be CD34- bright. Eighty-five to ninety percent were negative for three antigens expressed at an early stage of stem cell maturation: CD38, HLA-DR and LFA-1. Fifty-five percent of these CD34 (brig...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H E BroxmeyerP Rubinstein
Jan 1, 1993·Journal of Hematotherapy·C I Civin, S D Gore
Jan 1, 1993·Journal of Hematotherapy·M A Moore
May 1, 1993·The Journal of Experimental Medicine·W CraigP M Lansdorp
Jan 1, 1993·Journal of Hematotherapy·R TraineauE Gluckman
May 1, 1994·British Journal of Haematology·A J NicolB A Bradley

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Citations

Apr 20, 2000·Indian Journal of Pediatrics·S Inoue
Jan 19, 2002·Seminars in Hematology·Norbert Schmitz, John Barrett
Mar 27, 1999·Human Gene Therapy·A WinklerR G Andrews
Jun 14, 2003·Journal of Hematotherapy & Stem Cell Research·Nicholas J GrecoGary Moroff
Apr 27, 2017·Expert Opinion on Biological Therapy·Svitlana Garbuzova-DavisPaul R Sanberg
Mar 17, 2018·Molecular Systems Biology·Shiwei ZhengRahul Satija

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