A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation.

Nucleic Acids Research
Nikolay KorolevLars Nordenskiöld

Abstract

We report a systematic study of the condensation of plasmid DNA by oligocations with variation of the charge, Z, from +3 to +31. The oligocations include a series of synthetic linear ε-oligo(L-lysines), (denoted εKn, n = 3–10, 31; n is the number of lysines with the ligand charge Z = n+1) and branched α-substituted homologues of εK10: εYK10, εLK10 (Z = +11); εRK10, εYRK10 and εLYRK10 (Z = +21). Data were obtained by light scattering, UV absorption monitored precipitation assay and isothermal titration calorimetry in a wide range concentrations of DNA and monovalent salt (KCl, CKCl). The dependence of EC50 (ligand concentration at the midpoint of DNA condensation) on C(KCl) shows the existence of a salt-independent regime at low C(KCl) and a salt-dependent regime with a steep rise of EC50 with increase of C(KCl). Increase of the ligand charge shifts the transition from the salt-independent to salt-dependent regime to higher C(KCl). A novel and simple relationship describing the EC50 dependence on DNA concentration, charge of the ligand and the salt-dependent dissociation constant of the ligand–DNA complex is derived. For the ε-oligolysines εK6–εK10, the experimental dependencies of EC50 on C(KCl) and Z are well-described by an e...Continue Reading

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Citations

Aug 1, 1990·Journal of Biomolecular Structure & Dynamics·T WeidlichG A Thomas
Sep 24, 2013·Advances in Colloid and Interface Science·Nikolay V BerezhnoyLars Nordenskiöld
Jul 11, 2013·Nanoscale·Ting ZhouYanlian Yang
May 29, 2015·Nucleic Acids Research·Yuan-Yan WuZhi-Jie Tan
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Apr 28, 2016·Biophysical Journal·Nikolay V BerezhnoyLars Nordenskiöld
Feb 26, 2016·Scientific Reports·Yves LansacEric Raspaud
Jan 26, 2018·Scientific Reports·Nikolay KorolevLars Nordenskiöld
Jan 8, 2015·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Alexander P LyubartsevLars Nordenskiöld
Oct 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·T J ThomasC K S Pillai
May 21, 2019·Nucleic Acids Research·Tiedong SunLars Nordenskiöld
Jul 27, 2017·The Journal of Physical Chemistry. B·Tiedong SunLars Nordenskiöld

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