A Universal Influenza Virus Vaccine Candidate Tested in a Pig Vaccination-Infection Model in the Presence of Maternal Antibodies

Vaccines
Sun-Young SunwooJuergen A Richt

Abstract

The antigenically conserved hemagglutinin stalk region is a target for universal influenza virus vaccines since antibodies against it can provide broad protection against influenza viruses of different subtypes. We tested a universal influenza virus vaccination regimen based on sequential immunization with chimeric hemagglutinin (HA) containing viruses in a swine influenza virus pig model with maternal antibodies against pandemic H1N1. Vaccines were administered as live attenuated virus or inactivated influenza virus split vaccine (+/- Emulsigen adjuvant). As controls, we included groups that received trivalent inactivated influenza vaccine that contained pandemic H1N1 antigens, inactivated adjuvanted H1N2 vaccine (control group for vaccine associated enhanced respiratory disease in the pig model) or mock-vaccination. No induction of H1 head or stalk-specific antibody responses was observed upon vaccination, while responses against H3 and influenza B HA were elicited in the group vaccinated with the trivalent vaccine. Four weeks post vaccination, pigs were intratracheally challenged with pandemic H1N1 virus and euthanized 5 days after challenge. Despite the lack of detectable anti-stalk immunity, the chimeric hemagglutinin vacc...Continue Reading

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Citations

Apr 6, 2019·Frontiers in Immunology·Carolyn M Boudreau, Galit Alter
Feb 26, 2019·Frontiers in Immunology·Barbara HolzerElma Tchilian
Jan 15, 2022·Annals of the New York Academy of Sciences·Jennifer CableChristopher L Karp

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Methods Mentioned

BETA
ELISA
flow cytometry

Software Mentioned

VAERD
ChromaCal
Graphpad Prism
FlowJo
ImmunoCapture
GraphPad

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