A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis

Scientific Reports
Felix J HartmannSean C Bendall

Abstract

Single-cell barcoding enables the combined processing and acquisition of multiple individual samples as one. This maximizes assay efficiency and eliminates technical variability in both sample preparation and analysis. Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types. To that end, we developed a novel antibody-based platform that allows the robust barcoding of live human cells for mass cytometry (CyTOF). By targeting both the MHC class I complex (beta-2-microglobulin) and a broadly expressed sodium-potassium ATPase-subunit (CD298) with platinum-conjugated antibodies, human immune cells, stem cells as well as tumor cells could be multiplexed in the same single-cell assay. In addition, we present a novel palladium-based covalent viability reagent compatible with this barcoding strategy. Altogether, this platform enables mass cytometry-based, live-cell barcoding across a multitude of human sample types and provides a scheme for multiplexed barcoding of human single-cell assays in general.

References

Jun 17, 1998·The Journal of Biological Chemistry·A I IvanovP J Sadler
Oct 4, 2011·Nature Biotechnology·Peng QiuSylvia K Plevritis
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Stephen B WillinghamIrving L Weissman
May 12, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Harris G FienbergBernd Bodenmiller
Aug 21, 2012·Nature Biotechnology·Bernd BodenmillerGarry P Nolan
Mar 21, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rachel FinckSean C Bendall
Apr 2, 2013·Trends in Molecular Medicine·Ana Martin-VillalbaDamian Wollny
Jul 6, 2014·Experimental Hematology·Shalin H NaikLeïla Perié
Jan 23, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Henrik E MeiHolden T Maecker
Jan 24, 2015·Science·Mathias UhlénFredrik Pontén
Apr 22, 2015·PloS One·Damaris Bausch-FluckBernd Wollscheid
Jul 19, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cariad Chester, Holden T Maecker
Sep 12, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Henrik E MeiHolden T Maecker
Nov 10, 2015·European Journal of Immunology·Florian MairBurkhard Becher
May 8, 2016·Cell·Matthew H Spitzer, Garry P Nolan
Jun 21, 2016·Nature Reviews. Immunology·Yvan SaeysBart N Lambrecht
Nov 1, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hermi R SumatohEvan W Newell
Nov 9, 2016·The Journal of Experimental Medicine·Felix J HartmannBurkhard Becher
Nov 23, 2016·Cytometry. Part B, Clinical Cytometry·Quentin BacaBrice Gaudilliere
Jan 8, 2017·Methods in Molecular Biology·Dunja MrdjenBurkhard Becher
Jun 21, 2017·Scientific Reports·Ryan L McCarthyMichelle C Barton
Jan 9, 2018·Nature Medicine·Carsten KriegBurkhard Becher

❮ Previous
Next ❯

Citations

Oct 3, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Lars R OlsenHolden Terry Maecker
Dec 15, 2018·The FEBS Journal·Akshitkumar M MistryJonathan M Irish
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Apr 17, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Marie BurnsHenrik E Mei
Feb 16, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Lauren E HigdonJonathan S Maltzman
Apr 24, 2020·Computational and Structural Biotechnology Journal·Paulina RybakowskaConcepción Marañón
Jul 18, 2019·Cell Reports·Felix J HartmannSean C Bendall
Nov 24, 2020·European Journal of Immunology·Silje Michelsen SolbergSilke Appel
Oct 16, 2020·Brain Pathology·Camila Fernández-ZapataChotima Böttcher
Jan 22, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Raymond D DevineGregory K Behbehani
Jul 16, 2020·Immunity·David R GlassSean C Bendall
Apr 3, 2021·Immunology and Cell Biology·Felix Md Marsh-WakefieldRoslyn A Kemp
Jun 13, 2021·Scientific Reports·Muharrem MuftuogluMichael Andreeff
Jul 13, 2021·Frontiers in Immunology·Susan WestfallGiulio M Pasinetti
Jul 17, 2021·Nature Reviews. Cardiology·Dawn M Fernandez, Chiara Giannarelli
May 20, 2021·Cancer Immunology Research·Gyanu ParajuliShigeru Hashimoto
Jun 23, 2020·Bioconjugate Chemistry·Laura GrenierRaphaël Tripier
Dec 16, 2020·Analytical Chemistry·Antonio Delgado-Gonzalez, Rosario M Sanchez-Martin
Apr 6, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Chandresh R GajeraThomas J Montine

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
xenograft
blood donation
density gradient centrifugation

Software Mentioned

matlab
Adobe
Illustrator
R
SPADE
biorender
Cytobank

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Lisa M WillisMark Nitz
The Journal of Immunology : Official Journal of the American Association of Immunologists
Billur AkkayaMunir Akkaya
© 2022 Meta ULC. All rights reserved