A universal quantification of transgenic soybean event DAS-68416-4 using duplex digital PCR.

Journal of the Science of Food and Agriculture
Jin LiuDong-Wei Gao

Abstract

As labeling thresholds and low level presence thresholds of genetically modified (GM) components are implemented in more and more countries and regions, the demands for accurate quantification are increasing rapidly. At the same time, digital polymerase chain reaction (PCR) showed considerable benefits compared with former real-time fluorescence PCR in GM component quantification. A universal quantification method using duplex digital PCR was established for detection of transgenic soybean event DAS-68416-4. The absolute limits of quantification (LOQs) of DAS-68416-4 event-specific gene and lectin reference gene were 0.61 copies μL-1 and 4.6 copies μL-1 respectively in droplet digital PCR, while 0.522 copies μL-1 and 5.192 copies μL-1 in chip digital PCR. The relative LOQs of DAS-68416-4 percentage content was 0.1% in both two digital PCR systems. Gene copy ratio is the universal means of expression internationally used in transgenic component contents. Digital polymerase chain reaction (PCR) executes absolute quantification on specific genes, thus is considered to be suitable for detection of transgenic component contents. It was proved in this research on transgenic soybean event DAS-68416-4. Results indicated perfect satisfa...Continue Reading

References

Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, K W Kinzler
Oct 10, 2009·Analytical and Bioanalytical Chemistry·Philippe CorbisierKerry R Emslie
Nov 30, 2011·Analytical Chemistry·Leonardo B PinheiroKerry R Emslie
Dec 28, 2016·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Keita TsukaharaKazumi Kitta

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Citations

Dec 4, 2020·Biology·Caterina MorciaValeria Terzi

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