A universal radiochemical high-performance liquid chromatographic assay for the determination of UDP-glucuronosyltransferase activity

Analytical Biochemistry
Brian T EthellB Burchell

Abstract

A new unified assay for the determination of UDP-glucuronosyltransferase (UGT) activities has been developed. The resolution of [14C]uridine diphosphate glucuronic acid from radiolabeled glucuronides formed by incorporation of this radiolabel can now be achieved by a sensitive and rapid-gradient HPLC method which utilizes a radioactivity endpoint as a universal detection method. One important application of this method is the determination of kinetic parameters for cloned and expressed UGT isoforms with greater speed and precision than can be afforded by TLC methodology. Moreover, assays with 14C-labeled substrates indicate that gradient HPLC can easily resolve the substrate from the glucuronide products and present an alternative to the time-consuming optimization of conditions for organic phase extraction assays.

References

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Citations

Jun 9, 1999·The Biochemical Journal·G JedlitschkyB Burchell
Jul 20, 2007·Assay and Drug Development Technologies·O V TrubetskoyV S Trubetskoy
Dec 9, 2003·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·B K GaiserB Burchell
Dec 20, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yukiko SakakibaraMasayuki Nadai
Sep 11, 2010·Journal of Pharmaceutical and Biomedical Analysis·Si-Cheng LiangLing Yang
Jul 23, 2008·The Journal of Pharmacy and Pharmacology·Olga TrubetskoyVladimir Trubetskoy
Jun 24, 2003·Rapid Communications in Mass Spectrometry : RCM·Zhengyin YanJohn A Masucci
Oct 7, 2003·European Journal of Drug Metabolism and Pharmacokinetics·Z Yan, G W Caldwell
Sep 2, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Alison M MilneMichael W H Coughtrie
Mar 22, 2002·The Journal of Pharmacology and Experimental Therapeutics·M G SoarsR J Riley
Aug 5, 2004·The Journal of Pharmacology and Experimental Therapeutics·Adam G StainesBrian Burchell
Sep 28, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yoshihiro UesawaBrian Burchell
Jul 18, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Brian T EthellBrian Burchell
Jan 17, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Laurence AntonioJacques Magdalou
Aug 9, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Kenneth F IlettGeoffrey Edwards
May 23, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Brian T EthellBrian Burchell
Mar 25, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Annalise Di MarcoRalph Laufer
Jan 10, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M G SoarsB Burchell
Jul 5, 2001·Archives of Biochemistry and Biophysics·M G SoarsB Burchell
Feb 17, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·P LautalaJ Taskinen
Mar 12, 2003·Journal of Pharmacological and Toxicological Methods·Matthew G SoarsSteven A Wrighton

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