A universal type IA topoisomerase fold

Journal of Molecular Biology
M DuguetC Bouthier de la Tour

Abstract

A class of enzymes, called DNA topoisomerases, is responsible for controlling the topological state of cellular DNA. Among these, type IA topoisomerases form a vast family that is present in all living organisms, including higher eukaryotes, in which they play important roles in genome stability. The known 3D structures of three of these enzymes indicate that they share a common toroidal architecture. We previously showed that the toroidal structure could be split off from the core enzyme of Thermotoga maritima topoisomerase I by limited proteolysis. This structure is produced by the association of two tandemly repeated elementary folds in a head-to-tail orientation. By using a combination of structural and sequence data analysis, we show that the elementary fold of about 150 amino acid residues, referred to as the topofold, is likely to be present in the whole topoisomerase IA family. Within each enzyme, the successive topofolds share two conserved sequence motifs located at the base of the ring, and referred to as the MI and MII motifs. However, the overall sequences of the folds have largely diverged. By contrast, secondary and tertiary structures appear remarkably conserved. We suggest that this twofold repeat has evolved b...Continue Reading

References

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Citations

Aug 30, 2008·Quarterly Reviews of Biophysics·Allyn J Schoeffler, James M Berger
Feb 11, 2014·Nature Structural & Molecular Biology·Nicolas BocquetNicolas H Thomä
Dec 18, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Rafael Balaña-FouceRosa M Reguera
Apr 10, 2007·Biochimie·Jean-Michel RossignolPaola B Arimondo
Feb 13, 2007·Biochimie·Patrick ForterreMarie-Claude Serre
Dec 5, 2006·Biochimie·Thierry Viard, Claire Bouthier de la Tour
Jan 9, 2008·Molecular Microbiology·Jane R Scocca, Theresa A Shapiro
Dec 19, 2017·Nucleic Acids Research·Anna H BizardMarc Nadal
May 18, 2021·Journal of Molecular Evolution·Filipa MoreiraFilipe Pereira

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