A UPLC-MS/MS application for profiling of intermediary energy metabolites in microdialysis samples--a method for high-throughput

Journal of Pharmaceutical and Biomedical Analysis
Rune Isak Dupont BirklerMogens Johannsen

Abstract

Research within the field of metabolite profiling has already illuminated our understanding of a variety of physiological and pathological processes. Microdialysis has added further refinement to previous models and has allowed the testing of new hypotheses. In the present study, a new ultra-performance liquid chromatography/electrospray-tandem mass spectrometry (UPLC-ESI-MS/MS) method for the simultaneous detection and quantification of intermediary energy metabolites in microdialysates was developed. The targeted metabolites were mainly from the citric acid cycle in combination with pyruvic acid, lactic acid, and the ATP (adenosine triphosphate) hydrolysis product adenosine along with metabolites of adenosine. This method was successfully applied to analyze the microdialysates obtained from an experimental animal study giving insight into the hitherto unknown concentration of many interstitial energy metabolites, such as succinic acid and malic acid. With a total cycle time of 3 min, injection to injection, this method permits analysis of a much larger number of samples in comparison with conventional high performance liquid chromatography/tandem mass spectrometry HPLC-MS/MS strategies. With this novel combination where micro...Continue Reading

References

Dec 14, 2005·Circulation·Marc S SabatineRobert E Gerszten
Nov 21, 2008·Journal of Pharmaceutical and Biomedical Analysis·Ralph Nii Okai Tettey-Amlalo, Isadore Kanfer

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Citations

Jul 23, 2014·The Tohoku Journal of Experimental Medicine·Othoniel Hugo Aragon-MartinezFlavio Martinez-Morales
Mar 13, 2012·Cell Metabolism·Houman AshrafianHugh Watkins
Mar 31, 2017·The Journal of Biological Chemistry·María Julia BarisónMaria Belen Cassera
Jun 22, 2010·Cardiovascular Research·Nicolaj Brejnholt StøttrupTorsten Toftegaard Nielsen
Oct 16, 2016·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Janus Adler HyldebrandtHanne Berg Ravn
Dec 26, 2015·The Journal of Biological Chemistry·Tuomas LaukkaPeppi Koivunen
Nov 18, 2019·Biochimica Et Biophysica Acta. General Subjects·Lynne RumpingJudith J M Jans
Mar 11, 2021·Basic Research in Cardiology·Thomas Ravn LassenHans Erik Bøtker
May 7, 2021·Scientific Reports·Jacob Marthinsen SeefeldtHans Erik Bøtker
Aug 2, 2011·Journal of Proteome Research·Liyan ChenRoger W Beuerman

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