A user-friendly, highly sensitive assay to detect the IFN-gamma secretion by T cells.

Clinical Biochemistry
Paul L A M CorstjensAnnemiek Geluk

Abstract

Development of a user-friendly test alternative to ELISA-based assays to detect IFN-gamma by in vitro cultured peripheral blood mononuclear cells (PBMC) stimulated with pathogen-derived antigens. The molecular components of an operational IFN-gamma ELISA-based test were applied in a lateral flow (LF) immuno-sandwich assay using up-converting phosphor (UCP) reporter particles. The analytical sensitivity of the UCP-LF IFN-gamma assay (ULIGA) was determined and the assay was qualitatively validated with a selection of 60 supernatants derived from PBMC cultures stimulated with M. leprae derived antigens, mitogen or medium alone. ULIGA indicated an analytical sensitivity better than 2 pg/mL, and demonstrated four orders of magnitude dynamic range. The assay correlated well with the IFN-gamma ELISA. ULIGA allows detection well below the cutoff value (100 pg/mL) used to define positive responses in the IFN-gamma ELISA. The test procedure is less demanding in respect to equipment and labor, and is suited for testing single samples.

References

Dec 1, 1993·The Journal of Experimental Medicine·J L FlynnB R Bloom
Dec 26, 1996·The New England Journal of Medicine·M J NewportM Levin
Jan 31, 2006·IEE Proceedings. Nanobiotechnology·P L A M CorstjensH J Tanke
Apr 17, 2007·Annals of the New York Academy of Sciences·Vijaya K MokkapatiPaul L A M Corstjens
Oct 19, 2007·Journal of Clinical Microbiology·Paul L A M CorstjensGovert J van Dam

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Citations

Jul 15, 2009·Biomedical Microdevices·Xianbo QiuHaim H Bau
Jul 6, 2014·PLoS Neglected Tropical Diseases·Paul L A M CorstjensUNKNOWN Cysticercosis Working Group in Peru
Jun 4, 2014·Analytica Chimica Acta·Matthew V DaCostaUlrich J Krull
Jul 10, 2013·Analytical and Bioanalytical Chemistry·Paul L A M CorstjensHans J Tanke
Mar 30, 2013·Expert Opinion on Medical Diagnostics·Annemieke Geluk
Nov 30, 2011·Sensors and Actuators. B, Chemical·Xianbo QiuHaim H Bau
Jan 10, 2017·ACS Applied Materials & Interfaces·Zhiqin LiangLei Zhou
Oct 24, 2013·Nanomaterials·Xian ChenFeng Wang
Apr 1, 2016·Clinical and Vaccine Immunology : CVI·Paul L A M CorstjensAnnemieke Geluk
Sep 25, 2012·Nanoscale·Huichen Guo, Shiqi Sun
Jan 30, 2009·Clinical and Vaccine Immunology : CVI·Annemieke GelukUNKNOWN IDEAL Consortium
Jan 1, 2008·Revista Da Sociedade Brasileira De Medicina Tropical·Mariane Martins de Araújo Stefani
Apr 17, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Annemieke GelukTom H M Ottenhoff
Feb 13, 2016·PLoS Neglected Tropical Diseases·Edith Roset BahmanyarAnnemieke Geluk
Oct 25, 2020·Nature Protocols·Claudio ParoloArben Merkoçi
Feb 13, 2021·Proteomes·Sakshi GautamDeepa Bisht
Dec 5, 2018·Chemical Reviews·Christine F MarkwalterDavid W Wright

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