A user-friendly robotic sample preparation program for fully automated biological sample pipetting and dilution to benefit the regulated bioanalysis

Journal of Laboratory Automation
Hao JiangMark E Arnold

Abstract

Biological sample dilution is a rate-limiting step in bioanalytical sample preparation when the concentrations of samples are beyond standard curve ranges, especially when multiple dilution factors are needed in an analytical run. We have developed and validated a Microsoft Excel-based robotic sample preparation program (RSPP) that automatically transforms Watson worklist sample information (identification, sequence and dilution factor) to comma-separated value (CSV) files. The Freedom EVO liquid handler software imports and transforms the CSV files to executable worklists (.gwl files), allowing the robot to perform sample dilutions at variable dilution factors. The dynamic dilution range is 1- to 1000-fold and divided into three dilution steps: 1- to 10-, 11- to 100-, and 101- to 1000-fold. The whole process, including pipetting samples, diluting samples, and adding internal standard(s), is accomplished within 1 h for two racks of samples (96 samples/rack). This platform also supports online sample extraction (liquid-liquid extraction, solid-phase extraction, protein precipitation, etc.) using 96 multichannel arms. This fully automated and validated sample dilution and preparation process has been applied to several drug devel...Continue Reading

References

Dec 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·J Y HsiehM R Dobrinska
Apr 16, 1998·Journal of Pharmaceutical and Biomedical Analysis·A C Harrison, D K Walker
Mar 22, 2000·Journal of Pharmaceutical and Biomedical Analysis·N ZhangD T Rossi
Apr 20, 2004·Assay and Drug Development Technologies·Iris H XieHenry Y Wu
Jan 4, 2007·Assay and Drug Development Technologies·Huidong GuYuzhong Deng
Jan 30, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ji MaBradley K Wong

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Citations

Sep 5, 2015·Bioanalysis·Dong WeiLiyu Yang
Aug 1, 2014·Bioanalysis·Somaieh Soltani, Abolghasem Jouyban
Apr 19, 2011·The Journal of Molecular Diagnostics : JMD·Maurice ChanAnn Siew Gek Lee
Nov 2, 2016·Analytical Chemistry·Pei-Han Liu, Pawel L Urban
Dec 10, 2019·Bioprocess and Biosystems Engineering·A ChristlerA Jungbauer
Jun 6, 2021·Analytica Chimica Acta·Pedro Lemos de AlmeidaLuciano Farias de Almeida

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Software Mentioned

JANUS Automated Workstation
Freedom EVO scripts
Visual Basic for Applications ( VBA )
EVOware
Watson
VBA
Excel
Freedom EVO

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