Abstract
Biological sample dilution is a rate-limiting step in bioanalytical sample preparation when the concentrations of samples are beyond standard curve ranges, especially when multiple dilution factors are needed in an analytical run. We have developed and validated a Microsoft Excel-based robotic sample preparation program (RSPP) that automatically transforms Watson worklist sample information (identification, sequence and dilution factor) to comma-separated value (CSV) files. The Freedom EVO liquid handler software imports and transforms the CSV files to executable worklists (.gwl files), allowing the robot to perform sample dilutions at variable dilution factors. The dynamic dilution range is 1- to 1000-fold and divided into three dilution steps: 1- to 10-, 11- to 100-, and 101- to 1000-fold. The whole process, including pipetting samples, diluting samples, and adding internal standard(s), is accomplished within 1 h for two racks of samples (96 samples/rack). This platform also supports online sample extraction (liquid-liquid extraction, solid-phase extraction, protein precipitation, etc.) using 96 multichannel arms. This fully automated and validated sample dilution and preparation process has been applied to several drug devel...Continue Reading
References
Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·J HempeniusD A Doornbos
Dec 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·J Y HsiehM R Dobrinska
Nov 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·J HempeniusC C Lin
Apr 16, 1998·Journal of Pharmaceutical and Biomedical Analysis·A C Harrison, D K Walker
Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·A PolettiniM Montagna
Nov 18, 1998·Journal of Chromatography. a·K KronkvistH Jaegfeldt
Mar 22, 2000·Journal of Pharmaceutical and Biomedical Analysis·N ZhangD T Rossi
May 7, 2002·Rapid Communications in Mass Spectrometry : RCM·Yuzhong DengSteve E Unger
Apr 20, 2004·Assay and Drug Development Technologies·Iris H XieHenry Y Wu
Jan 4, 2007·Assay and Drug Development Technologies·Huidong GuYuzhong Deng
Jan 30, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ji MaBradley K Wong
Citations
Jul 19, 2013·Bioanalysis·Jia DuoYan J Zhang
Mar 13, 2014·Bioanalysis·Fumin LiStephen A Ploch
Dec 10, 2013·Journal of Pharmaceutical and Biomedical Analysis·Naiyu ZhengMark E Arnold
Sep 5, 2015·Bioanalysis·Dong WeiLiyu Yang
Apr 16, 2016·Bioanalysis·Fumin LiRand Jenkins
Aug 1, 2014·Bioanalysis·Somaieh Soltani, Abolghasem Jouyban
Nov 12, 2014·Bioanalysis·Naiyu ZhengJianing Zeng
Jul 31, 2014·PloS One·Alexander NagyJitka Horníčková
Apr 19, 2011·The Journal of Molecular Diagnostics : JMD·Maurice ChanAnn Siew Gek Lee
Dec 4, 2014·Veterinary Parasitology·Martina GallusováDavid Modrý
Nov 2, 2016·Analytical Chemistry·Pei-Han Liu, Pawel L Urban
Nov 30, 2016·Cytotechnology·J C NeubauerH Zimmermann
Dec 12, 2012·Bioanalysis·Long YuanMark E Arnold
Dec 10, 2019·Bioprocess and Biosystems Engineering·A ChristlerA Jungbauer
Dec 11, 2019·Talanta·Decibel P ElpaPawel L Urban
Jun 6, 2021·Analytica Chimica Acta·Pedro Lemos de AlmeidaLuciano Farias de Almeida