A user's guide to the Arabidopsis T-DNA insertion mutant collections

Methods in Molecular Biology
Ronan C O'MalleyJoseph R Ecker

Abstract

The T-DNA sequence-indexed mutant collections contain insertional mutants for most Arabidopsis thaliana genes and have played an important role in plant biology research for almost two decades. By providing a large source of mutant alleles for in vivo characterization of gene function, this resource has been leveraged thousands of times to study a wide range of problems in plant biology. Our primary goal in this chapter is to provide a general guide to strategies for the effective use of the data and materials in these collections. To do this, we provide a general introduction to the T-DNA insertional sequence-indexed mutant collections with a focus on how best to use the available data sources for good line selection. As isolation of a homozygous line is a common next step once a potential disruption line has been identified, the second half of the chapter provides a step-by-step guide for the design and implementation of a T-DNA genotyping pipeline. Finally, we describe interpretation of genotyping results and include a troubleshooting section for common types of segregation distortions that we have observed. In this chapter we introduce both basic concepts and specific applications to both new and more experienced users of t...Continue Reading

Citations

Apr 28, 2018·Journal of Experimental Botany·Natalia NikonorovaIve De Smet
Apr 18, 2018·Plant Cell Reports·Cynthia K Holland, Joseph M Jez
Apr 10, 2020·International Journal of Molecular Sciences·Benedict C OfforLizelle A Piater
Apr 23, 2017·The Plant Journal : for Cell and Molecular Biology·Mon Mandy HsiaJohn P Vogel
Nov 7, 2018·Nature Ecology & Evolution·Liesje Mommer, Jasper van Ruijven
Oct 24, 2020·Current Genomics·Seenichamy Rathinam PrabhukarthikeyanSamantaray Sanghamitra
Apr 9, 2019·Cell·Richard S MarshallRichard D Vierstra
Mar 20, 2020·Cell·Claire D McWhiteEdward M Marcotte
Sep 3, 2017·Seminars in Cell & Developmental Biology·Jennifer A N BrophyJosé R Dinneny
Sep 19, 2020·Current Biology : CB·Andrew MuroyamaDominique C Bergmann

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