A Vaccine Based on Kunitz-Type Molecule Confers Protection Against Fasciola hepatica Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production.

Frontiers in Immunology
Leonardo SilvaneLaura Cervi

Abstract

Fasciola hepatica is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the development of parasite resistance and, consequently, the limitation of its effectiveness. Hence, vaccination appears as an attractive option to develop. In this work, we evaluated the potential of F. hepatica Kunitz-type molecule (FhKTM) as an antigen formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) during an experimental model of fasciolosis in mice, and we further dissected the immune response associated with host protection. Our results showed that immunization of mice with FhKTM/CpG-ODN/Coa-ASC16 induces protection against F. hepatica challenge by preventing liver damage and improving survival after F. hepatica infection. FhKTM/CpG-ODN/Coa-ASC16-immunized mice elicited potent IFN-γ and IL-17A with high levels of antigen-specific IgG1, IgG2a, and IgA serum antibodies. Strikingly, IL-17A bl...Continue Reading

References

Oct 1, 1995·Molecular and Biochemical Parasitology·S E BozasT W Spithill
Oct 30, 2007·International Journal of Pharmaceutics·Santiago PalmaDaniel Allemandi
Aug 19, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sheila DonnellyJohn P Dalton
Apr 1, 2009·Infection and Immunity·Clare M HamiltonSandra M O'Neill
May 8, 2010·Veterinary Immunology and Immunopathology·Cristian FalcónLaura Cervi
Jan 8, 2013·International Journal for Parasitology·Henry J McSorleyRick M Maizels
Mar 20, 2013·Nature Immunology·Pierre J Milpied, Michael G McHeyzer-Williams
Dec 19, 2013·PLoS Pathogens·André Gladiator, Salomé LeibundGut-Landmann
Jan 3, 2014·Biomaterials·María F Sánchez VallecilloBelkys A Maletto
Jul 10, 2014·Mucosal Immunology·K Trautwein-WeidnerS LeibundGut-Landmann
Sep 10, 2014·International Journal for Parasitology·Hayley ToetTerry W Spithill
Oct 3, 2014·The Journal of Infectious Diseases·Manuela Sales Lima NascimentoJoão Santana da Silva
Feb 7, 2015·Veterinary Parasitology·Verónica Molina-HernándezKrystyna Cwiklinski
Dec 8, 2015·Parasitology Research·Verónica NoyaTeresa Freire
Dec 18, 2015·International Journal of Molecular Medicine·Qiyue GaoZuyi Yuan
Jan 12, 2016·Human Vaccines & Immunotherapeutics·Fei SuWangxue Chen
Apr 7, 2016·Trends in Parasitology·Jane M KelleyTerry W Spithill
Apr 29, 2016·Clinical and Vaccine Immunology : CVI·John D Clements, Lucy C Freytag
Oct 26, 2016·Biochemical Pharmacology·Christopher P Karch, Peter Burkhard
Jul 17, 2016·The Journal of Biological Chemistry·David SmithJohn P Dalton
Jul 29, 2016·Molecular & Cellular Proteomics : MCP·Alessandra RavidàSandra M O'Neill
Aug 16, 2016·Veterinary Parasitology·Narin ChangklungmoaPornanan Kueakhai
Sep 10, 2016·Journal of Helminthology·C Carmona, J F Tort

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
FCS
ELISA
lavage
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Veterinary Record
J F ReidF W Jennings
Proceedings, Annual Meeting of the United States Animal Health Association
R F Hall, B Z Lang
Endoscopy
M Wang, D K Pleskow
© 2021 Meta ULC. All rights reserved