PMID: 7544248Aug 1, 1995Paper

A vaccine conjugate of 'ISCAR' immunocarrier and peptide epitopes of the E7 cervical cancer-associated protein of human papillomavirus type 16 elicits specific Th1- and Th2-type responses in immunized mice in the absence of oil-based adjuvants

Clinical and Experimental Immunology
R W TindleG J Fernando

Abstract

TraT protein, known as ISCAR (= Immunostimulatory Carrier), is one of a family of integral membrane proteins (Imps) of Escherichia coli representing powerful carrier molecules which when injected into experimental animals generate substantial antibody and T proliferative responses to molecules conjugated to it. We extend these findings to show that ISCAR functions to stimulate Th1- and Th2-type responses, including specific cytotoxic T cells and tumour protection. We report here that by conjugating to ISCAR a 19mer peptide containing linear B epitopes, a T helper (Th) epitope, and a H-2b-restricted T cytotoxic (CTL) epitope of E7 protein of human papillomavirus type 16 (HPV16), and immunizing C57B1/6 (H-2b) mice, we elicited (i) specific IgG2a and IgG1 antibodies; (ii) IL-2 and IL-4 production by specifically recalled lymph node cells in vitro; (iii) cytotoxic T lymphocytes which specifically killed both E7 peptide-pulsed, and whole E7 gene-transfected tumour target cells; and (iv) in vivo protection against an E7 gene-transfected tumour cell inoculum. These findings have implications for the design of vaccines to stimulate immune responses to endogenously processed target antigens (e.g. tumour-associated antigens) without the ...Continue Reading

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Citations

Jun 17, 1998·International Journal of Cancer. Journal International Du Cancer·M ReddishB M Longenecker
Oct 1, 1998·European Journal of Immunology·I H FrazerR W Tindle
Aug 1, 2000·Indian Journal of Clinical Biochemistry : IJCB·S KurellaD N Rao
Oct 1, 1996·Current Opinion in Immunology·R W Tindle
Jul 30, 2002·The Lancet Infectious Diseases·Pierre Vandepapelière
Feb 5, 2013·Schizophrenia Bulletin·Guglielmo LuccheseDarja Kanduc
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wenyi GuNigel A J McMillan
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Dec 22, 2009·Expert Review of Vaccines·Luis G Bermúdez-Humarán, Philippe Langella
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Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·I H FrazerP Lambert
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carsten WietheRobert Tindle
Dec 10, 2014·Journal of Medicinal Chemistry·Tzu-Yu LiuMariusz Skwarczynski
Nov 28, 2020·Current HIV Research·Narges Farahani KhojastehAzam Bolhassani

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