PMID: 9445047Jan 28, 1998Paper

A vaccinia virus late transcription factor copurifies with a factor that binds to a viral late promoter and is complemented by extracts from uninfected HeLa cells

Journal of Virology
C F WrightB W Oswald

Abstract

We have previously described a vaccinia virus late transcription factor, VLTF-X, which we found to be present in cells at early and late times in infection. In this study, transcription complementation assays were used to demonstrate that VLTF-X activity is also present in virion extracts and in the cytoplasm of uninfected HeLa cells. Mobility shift assays performed on various VLTF-X preparations revealed that a late promoter DNA-binding activity cochromatographed and cosedimented with VLTF-X activity. Competition experiments demonstrated that this binding was specific for the late promoter region of the probe and that late transcription was dramatically reduced by an oligonucleotide that blocked factor-DNA complex formation but was only minimally affected by an oligonucleotide that did not inhibit complex formation. These results suggest that a cellular factor may participate in vaccinia virus late transcription. These findings also confirm the requirement for VLTF-X and distinguish it from any of the previously described vaccinia virus late transcription factors, which have all been mapped to the viral genome. Finally, these studies also suggest that the biochemical role for VLTF-X may be in late promoter recognition.

References

Dec 20, 1989·Journal of Molecular Biology·A J Davison, B Moss
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C F Wright, B Moss
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R RosalesB Moss

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Citations

Oct 9, 1998·The Journal of Biological Chemistry·S K GunasingheC F Wright
Aug 15, 2003·The Journal of General Virology·Steven S Broyles
May 12, 2006·The Biochemical Journal·Patrícia N G SilvaCláudio A Bonjardim
Mar 2, 2005·Nature Reviews. Microbiology·Grant McFadden
Jul 11, 1998·Journal of Virology·P Sanz, B Moss
Jan 8, 2000·The Journal of Biological Chemistry·C A Lackner, R C Condit

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