A vacuolar v-t-SNARE complex, the predominant form in vivo and on isolated vacuoles, is disassembled and activated for docking and fusion

The Journal of Cell Biology
C UngermannW Wickner

Abstract

Homotypic vacuole fusion in yeast requires Sec18p (N-ethylmaleimide-sensitive fusion protein [NSF]), Sec17p (soluble NSF attachment protein [alpha-SNAP]), and typical vesicle (v) and target membrane (t) SNAP receptors (SNAREs). We now report that vacuolar v- and t-SNAREs are mainly found with Sec17p as v-t-SNARE complexes in vivo and on purified vacuoles rather than only transiently forming such complexes during docking, and disrupting them upon fusion. In the priming reaction, Sec18p and ATP dissociate this v-t-SNARE complex, accompanied by the release of Sec17p. SNARE complex structure governs each functional aspect of priming, as the v-SNARE regulates the rate of Sec17p release and, in turn, Sec17p-dependent SNARE complex disassembly is required for independent function of the two SNAREs. Sec17p physically and functionally interacts largely with the t-SNARE. (a) Antibodies to the t-SNARE, but not the v-SNARE, block Sec17p release. (b) Sec17p is associated with the t-SNARE in the absence of v-SNARE, but is not bound to the v-SNARE without t-SNARE. (c) Vacuoles with t-SNARE but no v-SNARE still require Sec17p/Sec18p priming, whereas their fusion partners with v-SNARE but no t-SNARE do not. Sec18p thus acts, upon ATP hydrolysis...Continue Reading

References

Dec 1, 1992·The Journal of Cell Biology·B ConradtW Wickner
Jan 1, 1992·International Review of Cytology·C K RaymondT H Stevens
Oct 1, 1991·Journal of General Microbiology·D S Gomes de MesquitaC L Woldringh
Feb 3, 1995·The Journal of Biological Chemistry·H T McMahon, T C Südhof
Nov 3, 1994·Nature·J E Rothman
Jul 1, 1994·The Journal of Cell Biology·A HaasW Wickner
Jul 21, 1994·Nature·S Ferro-Novick, R Jahn
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Apr 12, 1996·Science·J E Rothman, F T Wieland
Mar 1, 1996·The Journal of Cell Biology·S K SappersteinM G Waters
Mar 1, 1996·The Journal of Cell Biology·Z Xu, W Wickner
Aug 23, 1996·The Journal of Biological Chemistry·A BanerjeeT F Martin
Jan 1, 1996·Annual Review of Cell and Developmental Biology·S R Pfeffer
May 8, 1997·Nature·B J NicholsA Haas
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·P SlusarewiczW T Wickner
Jun 2, 1997·The Journal of Cell Biology·I PaekJ E Rothman
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·H OttoR Jahn
Aug 1, 1997·Current Opinion in Cell Biology·J C Hay, R H Scheller
Jan 7, 1998·The Journal of Cell Biology·C Barlowe
Sep 1, 1995·Trends in Cell Biology·A Morgan, R D Burgoyne
Jul 1, 1997·Trends in Cell Biology·T F Martin
Jul 15, 1988·Science·C Holden

❮ Previous
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Citations

Nov 7, 2008·Protoplasma·M Götte, T Lazar
Jan 3, 2012·Journal of Molecular Evolution·Gustavo Caetano-AnollésDerek Caetano-Anollés
Oct 8, 2003·Neuropharmacology·Maria F MatosThomas C Südhof
May 22, 2003·Progress in Biophysics and Molecular Biology·Catherine van VlietPaul A Gleeson
Nov 20, 1998·Brain Research Bulletin·X Zheng, J A Bobich
Oct 28, 1998·Journal of Physiology, Paris·N MorelG Shiff
Aug 28, 1998·Current Opinion in Cell Biology·L J Robinson, T F Martin
Apr 13, 2000·The European Journal of Neuroscience·M R MukhtarovF Vyskocil
Oct 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher StroupeWilliam Wickner
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hao Xu, William T Wickner
Aug 6, 2009·The Journal of Biological Chemistry·Joji Mima, William Wickner
Dec 10, 1999·Molecular Biology of the Cell·E Grote, P J Novick
Jun 8, 1999·Molecular Biology of the Cell·G Fischer von Mollard, T H Stevens
Jan 19, 2000·Molecular Biology of the Cell·M J LewisH R Pelham
Jun 16, 2001·Molecular Biology of the Cell·F WendlerS A Tooze
Jul 15, 2003·Molecular Biology of the Cell·Ken Sato, Akihiko Nakano
Jan 27, 2004·Molecular Biology of the Cell·Allen VolchukLelio Orci
May 25, 2007·Molecular Biology of the Cell·Christina Schindler, Anne Spang
Dec 21, 2007·Molecular Biology of the Cell·Marcin BarszczewskiReinhard Jahn
Apr 4, 2008·Molecular Biology of the Cell·Vincent J StaraiWilliam Wickner
May 27, 2011·Molecular Biology of the Cell·Lukas Krämer, Christian Ungermann
Apr 16, 1999·The EMBO Journal·M Otter-NilssonT Nilsson
Oct 13, 2001·The EMBO Journal·O MüllerA Mayer
Mar 13, 2002·The EMBO Journal·William Wickner
Jun 29, 2000·Annual Review of Biochemistry·R Jahn, T C Südhof
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Andreas Mayer
Jan 25, 2012·PLoS Biology·Kannan AlpadiChristopher Peters
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F SealsA Price
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·L H ChamberlainG W Gould
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C UngermannW Wickner
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·C UngermannZ Xu
Aug 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alexey J Merz, William T Wickner

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Methods Mentioned

BETA
immunoprecipitation
crossprecipitation

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