A validated high-performance liquid chromatographic method for the determination of glibenclamide in human plasma and its application to pharmacokinetic studies

Journal of Pharmaceutical and Biomedical Analysis
Ioannis Niopas, Athanasios C Daftsios


Glibenclamide is a potent second generation oral sulfonylurea antidiabetic agent widely used for the treatment of type II diabetes melitus. A rapid, sensitive, precise, accurate and specific HPLC assay for the determination of glibenclamide in human plasma was developed and validated. After addition of flufenamic acid as internal standard, the analytes were isolated from human plasma by liquid-liquid extraction. The method was linear in the 10-400 ng/ml concentration range (r > 0.999). Recovery for glibenclamide was greater than 91.5% and for internal standard was 93.5%. Within-day and between-day precision, expressed as the relative standard deviation (RSD%), ranged from 1.4 to 5.9% and 5.8 to 6.6%, respectively. Assay accuracy was better than 93.4%. The assay was used to estimate the pharmacokinetics of glibenclamide after oral administration of a 5 mg tablet of glibenclamide to 18 healthy volunteers.


Feb 12, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J L C M DorneA G Renwick
Oct 8, 2016·Scientific Reports·Chidchamai KewcharoenwongGanjana Lertmemongkolchai
Jul 14, 2007·Journal of Pharmaceutical and Biomedical Analysis·Hiren N MistriPranav S Shrivastav
Oct 5, 2018·Frontiers in Immunology·Chidchamai KewcharoenwongGanjana Lertmemongkolchai

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