A validated solid-phase extraction HPLC method for the simultaneous determination of the citrus flavanone aglycones hesperetin and naringenin in urine

Journal of Pharmaceutical and Biomedical Analysis
Feras Imad KanazeIoannis Niopas

Abstract

A simple, specific, precise, accurate, and robust HPLC assay for the simultaneous analysis of hesperetin and naringenin in human urine was developed and validated. Urine samples were incubated with beta-glucuronidase/sulphatase and the analytes were isolated by solid-phase extraction using C18 cartridges and separated on a C8 reversed phase column using a mixture of methanol/water/acetic acid (40:58:2, v/v/v) at 45 degrees C. The method was found to be linear in the 50-1200 ng/ml concentration range for both hesperetin and naringenin (r > 0.999). The accuracy of the method was greater than 94.8%, while the intra- and inter-day precision for hesperetin was better than 4.9 and 8.2%, respectively and for naringenin was better than 5.3 and 7.8%, respectively. Recovery for hesperetin, naringenin and internal standard 7-ethoxycoumarin was greater than 70.9%. The method has been applied for the determination of hesperetin and naringenin in urine samples obtained from a male volunteer following a single 300 mg oral dose of each of the corresponding flavanone glycosides hesperidin and naringin. The intra- and inter-day reproducibility through enzyme hydrolysis was less than 3.9% for both total (free + conjugated) hesperetin and naringen...Continue Reading

Citations

Oct 9, 2012·Journal of Chromatographic Science·Marwa A A Ragab
Dec 17, 2014·Chinese Journal of Integrative Medicine·Kanika PatelDinesh Kumar Patel
Sep 22, 2010·Phytotherapy Research : PTR·Hsiu-Mei ChiangKuo-Ching Wen
Mar 1, 2012·Biopharmaceutics & Drug Disposition·Menghua LiuWeiwei Su
Sep 27, 2005·Journal of Pharmaceutical and Biomedical Analysis·Jianming HuangXubing Huang
Sep 11, 2009·Journal of Pharmaceutical and Biomedical Analysis·Kahina Si-AhmedSalvatore Fanali
Oct 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lorena de Lima GlóriaDaniela Barros de Oliveira

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