A validated stability-indicating HPLC method for determination of brimonidine tartrate in BRI/PHEMA drug delivery systems

Chemistry Central Journal
Jianguo SunTaomin Huang

Abstract

A simple, rapid and accurate stability-indicating reverse phase high performance liquid chromatography (RP-HPLC) was developed and validated for the determination of brimonidine tartrate in brimonidine tartrate/poly(2-hydroxyethyl methacrylate) (BRI/PHEMA) drug delivery contact lenses and pharmaceutical formulations. Optimum chromatographic conditions for separating brimonidine tartrate from other impurities in the leaching liquor of BRI/PHEMA drug delivery contact lenses or pharmaceutical formulations have been achieved by using a Diamonsil C18 column (150 mm × 4.6 mm, 5 μm) as a stationary phase and a mixture solution of phosphate buffer (10 mM, pH3.5) containing 0.5% triethlamine and methanol (85:15, v/v) as a mobile phase at a flow rate of 1 mL/min. The theoretical plates for the brimonidine tartrate measurement were calculated to be 8360 when detection was performed at 246 nm using a diode array detector. The proposed method was validated in accordance with ICH guidelines with respect to linearity, accuracy, precision, robustness, specificity, limit of detection and quantitation. Regression analysis showed a good correlation (R(2) > 0.999) for brimonidine tartrate in the concentration range of 0.01-50 μg/mL. The peak purit...Continue Reading

References

Jul 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·A Acheampong, D D Tang-Liu
Mar 18, 2008·Journal of Glaucoma·Deepta Ghate, Henry F Edelhauser
Mar 25, 2008·Therapeutics and Clinical Risk Management·Louis B Cantor
Dec 26, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sherwin JiangJoel W Proksch
Apr 25, 2009·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Clyde L SchultzJanet O Mint
Feb 7, 2012·Drug Discovery Today·Himanshu Gupta, Mohammed Aqil
Mar 7, 2013·Investigative Ophthalmology & Visual Science·Koji KakisuTetsuo Tochikubo
Jul 24, 2013·Expert Opinion on Drug Delivery·Lokendrakumar C BenganiAnuj Chauhan
Jan 27, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·I M CarvalhoD C Ferreira
Mar 10, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Jun XiangJianjiang Xu
Mar 17, 2015·Current Drug Delivery·Hediye Moghadam OmranipourSeyed Ahmad Mohajeri
Apr 17, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sonia T HassibRawda M Sayed

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BETA
column chromatography

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