A variable-length PCR target protein of Ehrlichia chaffeensis contains major species-specific antibody epitopes in acidic serine-rich tandem repeats.

Infection and Immunity
Tian LuoJere W McBride

Abstract

Ehrlichia chaffeensis and E. canis have a small subset of tandem repeat (TR)-containing proteins that elicit strong host immune responses and are associated with host-pathogen interactions. In a previous study, we molecularly characterized a highly conserved 19-kDa major immunoreactive protein (gp19) of E. canis and identified the corresponding TR-containing ortholog variable-length PCR target (VLPT) protein in E. chaffeensis. In this study, the native 32-kDa VLPT protein was identified and the immunodeterminants defined in order to further understand the molecular basis of the host immune response to E. chaffeensis. Synthetic and/or recombinant polypeptides corresponding to various regions of VLPT were used to localize major antibody epitopes to the TR-containing region. Major antibody epitopes were identified in three nonidentical repeats (R2, R3, and R4), which reacted strongly with antibodies in sera from an E. chaffeensis-infected dog and human monocytotropic ehrlichiosis patients. VLPT-R3 and VLPT-R2 reacted most strongly with antibody, and the epitope was further localized to a nearly identical proximal 17-amino-acid region common between these repeats that was species specific. The epitope in R4 was distinct from that o...Continue Reading

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Citations

Oct 22, 2013·Microbes and Infection·Paige Selvy DunphyJere W McBride
Feb 1, 2011·Expert Reviews in Molecular Medicine·Jere W McBride, David H Walker
Jun 2, 2010·Clinics in Laboratory Medicine·Nahed IsmailJere W McBride
Feb 2, 2010·Microbes and Infection·Abdul WakeelJere W McBride
Nov 26, 2008·Veterinary Parasitology·Stephen K Chapes, Roman R Ganta
Oct 21, 2015·Ticks and Tick-borne Diseases·Daniel M AguiarJere W McBride
Dec 10, 2013·Journal of Proteomics·Angelique Florentinus-MefailoskiJohn G Marshall
Jun 16, 2016·Frontiers in Cellular and Infection Microbiology·Taslima T LinaJere W McBride
Oct 22, 2015·Annual Review of Microbiology·Yasuko Rikihisa
Sep 9, 2010·Expert Review of Vaccines·Jere W McBride, David H Walker
May 30, 2017·Frontiers in Cellular and Infection Microbiology·Tian LuoJere W McBride
Jan 30, 2018·Frontiers in Cellular and Infection Microbiology·Tierra R FarrisJere W McBride
May 28, 2019·Frontiers in Immunology·Tyler R TominelloNahed Ismail
Aug 6, 2020·International Journal of Molecular Sciences·Alessandra TorinaGuido Sireci
Apr 30, 2021·Frontiers in Immunology·LaNisha L PattersonJere W McBride

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