A variable number of tandem repeats result in polymorphic alpha -isopropylmalate synthase in Mycobacterium tuberculosis

Tuberculosis
Wimon Chanchaem, Prasit Palittapongarnpim

Abstract

A locus of variable number of the tandem repeat, VNTR4155, resides in the putative leuA gene, encoding for alpha -isopropylmalate synthase (alpha -IPMS) of Mycobacterium tuberculosis, a repeat that is unique to the bacterium. The objective was to determine whether the polymorphic VNTR4155 was translated and resulted in a polymorphic protein. The putative leuA gene of the M. tuberculosis H37Rv strain was cloned by PCR and expressed in a His-tagged form in Escherichia coli. The enzymatic properties of the purified protein were studied. The protein was used as an antigen to immunize rabbits. Soluble proteins of several strains of M. tuberculosis were examined by Western blot analysis. The polymorphism of VNTR4155 was due to the presence of different copy number of the 57-bp tandem repeat. The putative alpha -IPMS of various strains of M. tuberculosis had different sizes, varying directly with the length of their VNTR4155.

References

Jan 1, 1991·Analytical Biochemistry·S M Conyers, D A Kidwell
Jan 1, 1988·Methods in Enzymology·G B Kohlhaw
Jun 13, 1980·Biochimica Et Biophysica Acta·P R Roeder, G B Kohlhaw
Jun 1, 1993·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·P PalittapongarnpimW Panbangred
May 1, 1996·Infection and Immunity·F C BangeW R Jacobs
May 23, 1998·Journal of Clinical Microbiology·J MagdalenaC Locht
Jun 5, 1998·Microbiology·Richard Frothingham, Winifred A Meeker-O'Connell
May 15, 1999·Journal of Clinical Microbiology·R FrothinghamD L Williams
Dec 28, 1999·JAMA : the Journal of the American Medical Association·P J BifaniB N Kreiswirth
Jul 27, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·N Smittipat, P Palittapongarnpim

❮ Previous
Next ❯

Citations

Jul 19, 2006·Biochemistry·Luiz Pedro S de Carvalho, John S Blanchard
May 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nayden KoonEdward N Baker
Oct 7, 2005·Journal of Clinical Microbiology·Nat SmittipatPrasit Palittapongarnpim
Dec 15, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bingji MaYuan Ruan
Sep 10, 2005·Applied Microbiology and Biotechnology·C E DandieS M Thomas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.