A variant beta-tubulin isoform of Drosophila melanogaster (beta 3) is expressed primarily in tissues of mesodermal origin in embryos and pupae, and is utilized in populations of transient microtubules

Developmental Biology
M KimbleE C Raff

Abstract

The beta 3-tubulin gene of Drosophila melanogaster codes for a variant tubulin isoform which is expressed at two distinct times during development: (1) during midembryogenesis from 8-16 hr postfertilization, and (2) during the 4 days of pupal development. We have determined the spatial pattern of beta 3-tubulin expression by localizing the beta 3 mRNA in paraffin sections using a 3' message-specific RNA probe and by localizing the beta 3 protein using a polyclonal antibody specific for Drosophila beta 3-tubulin. During embryogenesis beta 3 is restricted to and is expressed in all of the developing muscles. During pupal development beta 3 is also expressed at high levels in developing adult muscles. In addition, early in pupal development beta 3 is expressed in the imaginal discs, while at later times beta 3 is expressed in the epidermal cells of the wing blade, the optic lobe, the ovaries, and the testes. The expression of beta 3 tubulin ceases by the end of pupal development in all of these tissues except the ovaries and testes where expression persists into the adult. In both developing muscles and wings our results indicate that beta 3-tubulin is utilized in populations of specialized but transient cytoskeletal microtubules ...Continue Reading

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Citations

Dec 25, 2007·Cell Motility and the Cytoskeleton·Ellen M PopodiElizabeth C Raff
Nov 21, 2000·Current Biology : CB·E C RaffF R Turner
May 15, 2013·PloS One·Isabelle DevambezLaurent Soustelle
Jan 1, 1993·Cell Motility and the Cytoskeleton·R RenthalR F Ludueña
Mar 30, 2017·Molecular and Cellular Neurosciences·Martin W BreussDavid A Keays
Jul 14, 2001·Molecular Biology of the Cell·H D HoyleE C Raff
Oct 28, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Verónica Muñoz-SorianoNuria Paricio
Jan 1, 1993·Cell Motility and the Cytoskeleton·J M Andreu, J M de Pereda
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Jan 22, 2008·Cell Motility and the Cytoskeleton·Henry D HoyleElizabeth C Raff
Feb 29, 2020·Nature Reviews. Molecular Cell Biology·Carsten Janke, Maria M Magiera
Jul 16, 2003·Cold Spring Harbor Symposia on Quantitative Biology·E M Small, P A Krieg
Jan 1, 1995·Developmental Genetics·H D HoyleE C Raff
Mar 3, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rakhi JattaniMonn Monn Myat
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Jan 3, 1997·Science·E C RaffF R Turner

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