PMID: 8587807Jan 1, 1996Paper

A vasoactive intestinal polypeptide-like protein excreted/secreted by Nippostrongylus brasiliensis and its effect on contraction of uninfected rat intestine

Parasitology
N Foster, D L Lee

Abstract

The 50-30 kDa fraction isolated from the excretory/secretory products (E/S) of the nematode Nippostrongylus brasiliensis significantly decreased the amplitude of contraction of segments of uninfected rat intestine when injected into the lumen of the segments maintained in an organ bath. Dot blot analysis of the fraction suggested that it was similar in immunoreactivity to porcine vasoactive intestinal polypeptide (VIP). When antiserum to porcine VIP was mixed with N. brasiliensis E/S and the mixtures were injected into the lumen of segments of rat intestine, the inhibitory effect of the E/S on amplitude of contraction decreased. When physiological concentrations of porcine VIP (12.9 pmol/ml) were injected into the lumen of segments of uninfected rat intestine the amplitude of contraction decreased significantly. Western blot analysis of the E/S, using antiserum to porcine VIP, recognized a 30 kDa protein in the E/S and also in whole worm homogenate suggesting that synthesis of the peptide occurs inside the nematode. Peptide histidine isoleucine (PHI)-like immunoreactivity was detected in a 68 kDa fraction of the E/S and the homogenate but this fraction did not affect the amplitude of contractions of the intestine.

References

Oct 6, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·K Turnheim, F Lauterbach
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 26, 1991·Regulatory Peptides·R S IzzoR S Lokchander
Jan 1, 1990·Gastroenterology·A I CrosthwaiteJ A Fox
Apr 15, 1990·The Journal of Comparative Neurology·P SithigorngulC Cowden

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Citations

May 1, 1996·International Journal for Parasitology·D L Lee
Feb 1, 1997·Parasitology Today·D M McKay, I Fairweather
Jan 1, 1996·Parasitology·D P ThompsonT G Geary
Dec 1, 1996·Immunology and Cell Biology·M RiffkinP Wood
Jun 28, 2001·The Journal of Parasitology·J M Palmer, B Greenwood-Van Meerveld
Jan 6, 2006·Parasitology·D M McKay
Jan 25, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C J FaroJ M Palmer

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