A ventrally localized protease in the Drosophila egg controls embryo dorsoventral polarity.

Current Biology : CB
Yong Suk ChoDavid Stein

Abstract

Drosophila embryo dorsoventral (DV) polarity is defined by serine protease activity in the perivitelline space (PVS) between the embryonic membrane and the inner layer of the eggshell. Gastrulation Defective (GD) cleaves and activates Snake (Snk). Activated Snk cleaves and activates Easter (Ea), exclusively on the ventral side of the embryo. Activated Ea then processes Spätzle (Spz) into the activating ligand for Toll, a transmembrane receptor that is distributed throughout the embryonic plasma membrane. Ventral activation of Toll depends upon the activity of the Pipe sulfotransferase in the ventral region of the follicular epithelium that surrounds the developing oocyte. Pipe transfers sulfate residues to several protein components of the inner vitelline membrane layer of the eggshell. Here we show that GD protein becomes localized in the ventral PVS in a Pipe-dependent process. Moreover, ventrally concentrated GD acts to promote the cleavage of Ea by Snk through an extracatalytic mechanism that is distinct from GD's proteolytic activation of Snk. Together, these observations illuminate the mechanism through which spatially restricted sulfotransferase activity in the developing egg chamber leads to localization of serine prote...Continue Reading

References

May 1, 1988·Developmental Biology·K D KonradA P Mahowald
Oct 22, 1982·Science·G M Rubin, A C Spradling
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·K D KonradJ L Marsh
Dec 22, 1998·Mechanisms of Development·P Rørth
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J H HanC Hashimoto
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·E K LeMosyC Hashimoto
Sep 27, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Siegfried Roth
Nov 8, 2005·Current Biology : CB·Bernard Moussian, Siegfried Roth
Apr 10, 2009·Protein and Peptide Letters·Sangeetha Sukumari-Ramesh, Ellen K LeMosy

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Citations

Oct 11, 2015·Biochimie·Florian VeillardJean-Marc Reichhart
Feb 2, 2013·Trends in Genetics : TIG·Ben-Zion ShiloNaama Barkai
Sep 14, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Erin J PlosaLawrence S Prince
Aug 16, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·David S Stein, Leslie M Stevens
Jan 23, 2019·Journal of Experimental Botany·Judith K Paulus, Renier A L Van der Hoorn
Jul 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Judith K PaulusRenier A L van der Hoorn
Apr 9, 2021·Biochemistry·Kundan KumarSiddhesh S Kamat

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