A versatile cancer cell trapping and 1D migration assay in a microfluidic device

Biomicrofluidics
Colin L HiseyDerek J Hansford

Abstract

Highly migratory cancer cells often lead to metastasis and recurrence and are responsible for the high mortality rates in many cancers despite aggressive treatment. Recently, the migratory behavior of patient-derived glioblastoma multiforme cells on microtracks has shown potential in predicting the likelihood of recurrence, while at the same time, antimetastasis drugs have been developed which require simple yet relevant high-throughput screening systems. However, robust in vitro platforms which can reliably seed single cells and measure their migration while mimicking the physiological tumor microenvironment have not been demonstrated. In this study, we demonstrate a microfluidic device which hydrodynamically seeds single cancer cells onto stamped or femtosecond laser ablated polystyrene microtracks, promoting 1D migratory behavior due to the cells' tendency to follow topographical cues. Using time-lapse microscopy, we found that single U87 glioblastoma multiforme cells migrated more slowly on laser ablated microtracks compared to stamped microtracks of equal width and spacing (p < 0.05) and exhibited greater directional persistence on both 1D patterns compared to flat polystyrene (p < 0.05). Single-cell morphologies also diff...Continue Reading

References

Aug 1, 1996·Neurosurgery·A Giese, M Westphal
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C Holland
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Mar 30, 2004·Biomaterials·Bangshang ZhuZongguang Wang
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Anita C BellailErwin G Van Meir
Apr 23, 2004·Current Opinion in Biotechnology·Helene Andersson, Albert van den Berg
Jul 13, 2006·Annual Review of Biomedical Engineering·Joel Voldman
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Dec 26, 2006·Analytical Chemistry·Dino Di Carlo, Luke P Lee
Oct 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·M PoujadeP Silberzan
Oct 30, 2007·Biomedical Microdevices·Liz Y WuLuke P Lee
Nov 28, 2007·Annual Review of Pathology·David N Louis
Aug 2, 2008·The New England Journal of Medicine·Patrick Y Wen, Santosh Kesari
Jan 6, 2009·Nature Methods·Alison M SkelleyJoel Voldman
Feb 10, 2009·Tissue Engineering. Part C, Methods·Jed JohnsonJohn J Lannutti
Feb 18, 2009·The Journal of Cell Biology·Andrew D DoyleKenneth M Yamada
Sep 23, 2011·Journal of Cellular Biochemistry·Kana KobayashiJunya Tanaka
Oct 5, 2011·Neoplasia : an International Journal for Oncology Research·Paula A Agudelo-GarciaMariano S Viapiano
Oct 19, 2011·The Journal of Pathology·Pavlo G GritsenkoPeter Friedl
Nov 29, 2011·Cell·Peter Friedl, Stephanie Alexander
Sep 1, 2012·Lab on a Chip·Daniel Gallego-PerezDerek J Hansford
Sep 4, 2012·Mutation Research·Nina KramerHelmut Dolznig
Feb 2, 2013·Lab on a Chip·Aaron ChenRatnasingham Sooryakumar
May 21, 2013·Nano Letters·Melikhan Tanyeri, Charles M Schroeder
Aug 30, 2013·Nature Reviews. Neuroscience·Lorraine W LauV Wee Yong
Sep 21, 2013·Tissue Engineering. Part B, Reviews·Shreyas S RaoJessica O Winter
Jan 1, 2011·Pharmaceutics·Keren I Hulkower, Renee L Herber
Jun 8, 2014·Biosensors & Bioelectronics·A Benavente-BabaceM Mujika
Jun 21, 2014·Nature Reviews. Neuroscience·Vishnu Anand CuddapahHarald Sontheimer
Jun 26, 2014·Molecular Cancer Research : MCR·Yushan Kim, Sanjay Kumar
Sep 18, 2014·Advanced Healthcare Materials·Junghwa ChaPilnam Kim
May 18, 2016·Scientific Reports·Alexander BeliveauAnjana Jain
Jun 2, 2016·Nutrition Research and Practice·Hyuck KimJin-Young Moon
Jun 14, 2016·Cell Reports·Chris L SmithAndre Levchenko
Sep 9, 2017·Future Science OA·Weikun XiaoStephanie K Seidlits
Nov 19, 2017·Annals of Biomedical Engineering·Horacio M EstabridisDavid J Odde

❮ Previous
Next ❯

Citations

Apr 25, 2021·Colloids and Surfaces. B, Biointerfaces·Colin L HiseyLawrence W Chamley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cancer Sequencing

Several sequencing approaches are employed to understand and examine tumor development and progression. These include whole genome as well as RNA sequencing. Here is the latest research on cancer sequencing.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.