A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics

Biophysical Journal
Sebastian TackeRudolf Reichelt

Abstract

Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.

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Citations

Apr 30, 2017·Journal of Synchrotron Radiation·V B MykhaylykH Kraus
Jul 31, 2019·Soft Matter·Daniel MessmerA Dieter Schlüter
May 11, 2021·Journal of Structural Biology·Sebastian TackeStefan Raunser

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