A versatile label-free and signal-on electrochemical biosensing platform based on triplex-forming oligonucleotide probe

Analytica Chimica Acta
Xiuzhong WangFeng Li

Abstract

Nucleic acid and protein assays are very important in modern life sciences, and the recently developed triplex-forming oligonucleotide probes provide a unique means for biological analysis of different kinds of analytes. Herein, we report a label-free and signal-on electrochemical sensor for the detection of specific targets, which is based on the triple-helix structure formation between the hairpin molecular beacon and the capture probe through the intermolecular DNA hybridization induced by Watson-Crick and Hoogsteen base pairings. Upon the introduction of a specific target, the triple-helical stem region is dissembled to liberate the hemin aptamer, and a G-quadruplex- hemin complex can be formed in the presence of K(+) and hemin on the electrode surface to give an electrochemical response, thus signaling the presence of the target. With the use of Human Immunodeficiency Virus type 1 (HIV-1) as a proof-of-principle analyte, we first demonstrated this approach by using a molecular beacon, which consists of a central section with the DNA sequence complementary to HIV-1, flanked by two arm segments. This newly designed protocol provides an ultrasensitive electrochemical detection of HIV-1 with a limit of detection down to 0.054 ...Continue Reading

References

Apr 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·P P Chan, P M Glazer
Oct 10, 2006·Chemical Communications : Chem Comm·Zoya CheglakovItamar Willner
May 31, 2007·Angewandte Chemie·Tom N GrossmannOliver Seitz
Jul 29, 2011·Analytical Chemistry·Jing ZhengWeihong Tan
Jun 22, 2012·Analytical Chemistry·Jiahao HuangZhigang Li
Nov 30, 2012·Journal of the American Chemical Society·Jing ZhengWeihong Tan
Dec 25, 2012·Biophysical Journal·Il-Buem LeeAlbert Johner
Jul 9, 2013·Analytica Chimica Acta·Liangliang HuangYunhui Yang

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Citations

Jan 12, 2017·The Analyst·Gabriel Antonio S MineroMikkel F Hansen
Apr 27, 2017·Angewandte Chemie·Yuwei HuItamar Willner

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