A versatile technique for the in vivo imaging of human tumor xenografts using near-infrared fluorochrome-conjugated macromolecule probes

PloS One
Hiroshi SuemizuMasato Nakamura

Abstract

Here, we present a versatile method for detecting human tumor xenografts in vivo, based on the enhanced permeability and retention (EPR) effect, using near-infrared (NIR) fluorochrome-conjugated macromolecule probes. Bovine serum albumin (BSA) and two immunoglobulins-an anti-human leukocyte antigen (HLA) monoclonal antibody and isotype control IgG2a-were labeled with XenoLight CF770 fluorochrome and used as NIR-conjugated macromolecule probes to study whole-body imaging in a variety of xenotransplantation mouse models. NIR fluorescent signals were observed in subcutaneously transplanted BxPC-3 (human pancreatic cancer) cells and HCT 116 (colorectal cancer) cells within 24 h of NIR-macromolecule probe injection, but the signal from the fluorochrome itself or from the NIR-conjugated small molecule (glycine) injection was not observed. The accuracy of tumor targeting was confirmed by the localization of the NIR-conjugated immunoglobulin within the T-HCT 116 xenograft (in which the orange-red fluorescent protein tdTomato was stably expressed by HCT 116 cells) in the subcutaneous transplantation model. However, there was no significant difference in the NIR signal intensity of the region of interest between the anti-HLA antibody gro...Continue Reading

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Citations

May 3, 2015·Molecular Immunology·Amanda C Freise, Anna M Wu
May 20, 2015·Nature Cell Biology·Bruno Costa-SilvaDavid Lyden
Apr 4, 2021·Journal of Clinical Medicine·Ruth González-GómezAlberto J Schuhmacher

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Methods Mentioned

BETA
xenograft
xenografts
Fluorescence
fluorescence imaging
genetic

Software Mentioned

Prism
Living Image

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