A versatile transreplication-based system to identify cellular proteins involved in geminivirus replication

Journal of Virology
Gabriel MorillaEduardo R Bejarano

Abstract

A versatile green fluorescent protein (GFP) expression cassette containing the replication origins of the monopartite begomovirus Tomato yellow leaf curl Sardinia virus (TYLCSV) is described. Transgenic Nicotiana benthamiana plants containing one copy of the cassette stably integrated into their genome were superinfected with TYLCSV, which mobilized and replicated the cassette as an episomal replicon. The expression of the reporter gene (the GFP gene) was thereby modified. Whereas GFP fluorescence was dimmed in the intercostal areas, an increase of green fluorescence in veins of all leaves placed above the inoculation site, as well as in transport tissues of roots and stems, was observed. The release of episomal trans replicons from the transgene and the increase in GFP expression were dependent on the cognate geminiviral replication-associated protein (Rep) and required interaction between Rep and the intergenic region of TYLCSV. This expression system is able to monitor the replication status of TYLCSV in plants, as induction of GFP expression is only produced in those tissues where Rep is present. To further confirm this notion, the expression of a host factor required for geminivirus replication, the proliferating cellular ...Continue Reading

References

Aug 11, 1992·Nucleic Acids Research·M C TimmermansJ Messing
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A G DayC P Lichtenstein
Jun 1, 1995·The Plant Journal : for Cell and Molecular Biology·D C BaulcombeS Santa Cruz
Jul 1, 1995·Transgenic Research·F A van EngelenW J Stiekema
Mar 25, 1995·Nucleic Acids Research·F Heyraud-NitschkeB Gronenborn
Jan 1, 1996·Journal of Virology·B M Orozco, L Hanley-Bowdoin
Dec 18, 1995·FEBS Letters·J LaufsB Gronenborn
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·S S CruzK J Oparka
Apr 8, 1998·Current Opinion in Genetics & Development·S Mittnacht
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·P M WaterhouseM B Wang
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·O VoinnetD C Baulcombe
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C F Chuang, E M Meyerowitz
Nov 1, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E Warbrick
Jan 4, 2001·Virus Research·E Moriones, J Navas-Castillo
Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·F RatcliffD C Baulcombe
Sep 5, 2001·The Plant Journal : for Cell and Molecular Biology·C PeeleD Robertson
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·S V WesleyP M Waterhouse
Mar 5, 2002·The Plant Journal : for Cell and Molecular Biology·Jack R PeartDavid C Baulcombe
Dec 20, 2002·Biotechnology and Bioengineering·Tsafrir S MorHugh S Mason
Feb 14, 2003·Journal of Virology·Werner Preiss, Holger Jeske
Jun 28, 2003·Methods : a Companion to Methods in Enzymology·Rui LuDavid C Baulcombe
Oct 29, 2003·Current Opinion in Biotechnology·Li Kim Lee, Charles M Roth
Jan 27, 2004·Veterinary Microbiology·Crisanto GutierrezRiccardo Missich
Mar 3, 2004·Journal of Virology·A G CastilloE R Bejarano
Jun 15, 2004·Journal of Virology·Bettina BöttcherHolger Jeske
Jul 21, 2004·Journal of Molecular Microbiology and Biotechnology·K L HefferonY S Moon

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Citations

Oct 2, 2007·Plant Physiology·Yuval PeretzIlan Sela
Jul 22, 2011·Journal of Virology·Miguel A Sánchez-DuránEduardo R Bejarano
Feb 18, 2014·Virus Research·Judith Bach, Holger Jeske
Jan 30, 2009·Plant Biotechnology Journal·Changjun HuangXueping Zhou
Feb 21, 2007·Plant Biotechnology Journal·Hervé VanderschurenPeng Zhang
Dec 25, 2009·The Journal of General Virology·Julia B ErdmannHolger Jeske
Jul 28, 2015·The New Phytologist·Rosa Lozano-Durán
Dec 3, 2014·Virus Research·Tobias PaprotkaHolger Jeske
Jul 20, 2010·Journal of Virological Methods·Björn KrenzHolger Jeske
May 27, 2016·Scientific Reports·Zahir AliMagdy M Mahfouz
Oct 8, 2016·Virology·Kathrin DeuschleHolger Jeske
Jan 16, 2015·Journal of Virology·Björn KrenzHolger Jeske
Oct 5, 2019·Phytopathology·Verónica Pérez-PadillaEnrique Moriones
Feb 27, 2017·Virus Genes·Björn KrenzSusanna Krapp
Aug 8, 2020·Frontiers in Plant Science·Francesca MaioHarrold A van den Burg

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